Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54274 Общая биофизика General biophysics Общая биофизика MICROSEPARATION CELL FOR FREE-FLOW ELECTROPHORESIS OF THE TARGET PROTEIN МИКРОСЕПАРАЦИОННАЯ ЯЧЕЙКА ДЛЯ ВЫДЕЛЕНИЯ ЦЕЛЕВОГО БЕЛКА МЕТОДОМ ЭЛЕКТРОФОРЕЗА В СВОБОДНОМ ПОТОКЕ Леонович В А Leonovich V A Сорокина О Н Sorokina O N alsiona@gmail.com Климов М А Klimov M A Пронкин П Г Pronkin P G Плащина И Г Plashchina I G Подойницын С Н Podoynitsyn S N Институт биохимической физики им. Н.М. Эмануэля РАН ru N.M. Emanuel Institute for Biochemical Physics RAS ru Институт биохимической физики им. Н.М. Эмануэля РАН ru N.M. Emanuel Institute for Biochemical Physics RAS ru Институт биохимической физики им. Н.М. Эмануэля РАН ru N.M. Emanuel Institute for Biochemical Physics RAS ru Институт биохимической физики им. Н.М. Эмануэля РАН ru N.M. Emanuel Institute for Biochemical Physics RAS ru Институт биохимической физики им. Н.М. Эмануэля РАН ru N.M. Emanuel Institute for Biochemical Physics RAS ru Институт биохимической физики им. Н.М. Эмануэля РАН ru N.M. Emanuel Institute for Biochemical Physics RAS ru 25 06 2018 25 06 2018 3 2 245 249 20 06 2018 20 06 2018 https://rusjbpc.ru/en/nauka/article/54274/view

Предложена конструкция упрощенного сепарационного устройства и методики для выделения целевого белка из смеси методом зонного электрофореза в свободном потоке. Эффективность устройства была продемонстрирована теоретически и экспериментально на примере сепарации белков гемоглобина и сывороточного альбумин человека. Предложенный метод сепарации отличается простотой использования и хорошей производительностью.

The construction of a simplified separation device and a method for isolating the target protein from a mixture of proteins by a free-flow electrophoresis method is proposed. The effectiveness of the device was theoretically and experimentally demonstrated on the example of separation of hemoglobin protein and human serum albumin. This method of separation is characterized by ease of use and good productivity.

 микросепарационная ячейка электрофорез microsection cell electrophoresis

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