Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54302 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ FUNCTIONAL CHANGES IN ANIMAL ERYTHROCYTES IN A CONSTANT ELECTRIC FIELD ФУНКЦИОНАЛЬНОЕ ИЗМЕНЕНИЕ ЭРИТРОЦИТОВ ЖИВОТНЫХ ПРИ ДЕЙСТВИИ ПОСТОЯННОГО ЭЛЕКТРИЧЕСКОГО ПОЛЯ Олешкевич А А Oleshkevich A A kompsotita@gmail.com Московская государственная академия ветеринарной медицины и биотехнологии им. К.И. Скрябина ru Moscow State Academy of Veterinary Medicine and Biotechnology ru 25 06 2018 25 06 2018 3 2 401 405 20 06 2018 20 06 2018 https://rusjbpc.ru/en/nauka/article/54302/view

Изучены эффекты постоянного электрического поля на функциональные особенности эритроцитов, проницаемость их мембран для глюкозы, динамику оксигенации гемоглобина, а также на изменение активности цитозольных ферментов. В зависимости от вида животных воздействие более 5 мин вызывало достоверное увеличение активности от 1,5 до 4 раз лактатдегидрогеназы КФ 1.1.1.27 и/или аспартатаминотрансферазы КФ 2.6.1.1 плазмы крови кошек, собак и лошадей. Регистрировали ряд физиологических деструктивных изменений форменных элементов крови: анизо- и пойкилоцитоз, увеличение площади тромбоцитов, агрегацию клеток, кариолизис, фрагментоз и др. Качественные и количественные изменения клеточных характеристик и параметров носили дозозависимый характер. Показано наличие видовых особенностей клеточного ответа.

The effects of a constant electric field on the functional features of erythrocytes, the permeability of their membranes for glucose, the dynamics of hemoglobin oxygenation, and the change in the activity of erythrocyte cytosolic enzymes were studied. Depending on the type of an animal, 5-minute and longer exposure caused 1.5-4-fold increase of lactate dehydrogenase KF 1.1.1.27 and/or aspartate aminotransferase KF 2.6.1.1 activity in blood plasma of cats, dogs and horses. Several physiological destructive changes in blood cells were recorded: aniso- and poikilocytosis, an increase in platelet area, aggregation of cells, karyolysis, fragmentogenesis, etc. Qualitative and quantitative changes in cellular characteristics and parameters were dose-dependent. The presence of specific features of the cellular response is shown.

 электростатическое поля биологические эффекты клетки крови ферменты electrostatic field biological effects blood cells enzymes

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