Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54305 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ THE ROLE OF GAS-TRANSMITTERS IN THE MECHANISMS OF PURINERGIC REGULATION OF THE CONTRACTIVE ACTIVITY OF VASCULAR GLADOMETER CELLS РОЛЬ ГАЗОТРАНСМИТТЕРОВ В МЕХАНИЗМАХ ПУРИНЕРГИЧЕСКОЙ РЕГУЛЯЦИИ СОКРАТИТЕЛЬНОЙ АКТИВНОСТИ СОСУДИСТЫХ ГЛАДКОМЫШЕЧНЫХ КЛЕТОК Смаглий Л В Smagliy L V lud.smagly@yandex.ru Рыдченко В С Rydchenko V S Ярцева Ю О Yartseva Yu O Бирулина Ю Г Birulina Yu G Ковалев Игорь Викторович Kovalev I. V.

доктор медицинских наук;

doctor of medical sciences;

 Гусакова С В Gusakova S V Носарев А В Nosarev A V Петрова И В Petrova I V Сибирский государственный медицинский университет Минздрава России ru SibSMU of the Ministry of Health of Russia ru Сибирский государственный медицинский университет Минздрава России ru SibSMU of the Ministry of Health of Russia ru Сибирский государственный медицинский университет Минздрава России ru SibSMU of the Ministry of Health of Russia ru Сибирский государственный медицинский университет Минздрава России ru SibSMU of the Ministry of Health of Russia ru Сибирский государственный медицинский университет Минздрава России Томск Россия SibSMU of the Ministry of Health of Russia Tomsk Russian Federation Сибирский государственный медицинский университет Минздрава России ru SibSMU of the Ministry of Health of Russia ru Сибирский государственный медицинский университет Минздрава России ru SibSMU of the Ministry of Health of Russia ru Сибирский государственный медицинский университет Минздрава России ru SibSMU of the Ministry of Health of Russia ru 25 06 2018 25 06 2018 3 2 416 421 20 06 2018 20 06 2018 https://rusjbpc.ru/en/nauka/article/54305/view

Исследовали роль сероводорода (H2S) в действии агониста P2X и P2Y рецепторов АТФ на тонус сегментов аорты крысы с интактным эндотелием. АТФ (1-1000 мкМ) и агонист P2X рецепторов α,β-MeATP (100 мкМ) расслабляли сегменты, предсокращенные активатором α1-адренорецепторов фенилэфрином (ФЭ, 10 мкМ). Агонист P2Y рецепторов УТФ (1-100 мкМ) снижал, а УТФ (100-1000 мкМ) - увеличивал амплитуду ФЭ-индуцированного сокращения. Релаксирующее действие АТФ усиливалось донором сероводорода NaHS (500 мкМ), но снижалось в присутствии ингибитора цистотионин-γ-лиазы (CSE) DL-propargylglicine (10 мМ). DL-propargylglicine (10 мМ) увеличивал величину констрикторного действия УТФ (100-1000 мкМ), но снижал релаксирующее действие α,β-MeATP (100 мкМ). Таким образом, активация P2X рецепторов снижает величину сокращения, индуцированного фенилэфрином, а активация P2Y рецепторов - увеличивает. При этом релаксирующее действие АТФ на сегменты аорты крысы с интактным эндотелием обусловлено активацией цистотионин-γ-лиазы через P2X рецепторы.

The role of hydrogen sulfide (H2S) in ATP action on tone of rat aorta segments with intact endothelium was studied. ATP (1-1000 µM), a nonselective activator of P2X and P2Y receptors, relaxed vascular segments precontracted with agonist of α1-adrenergic receptors phenilephrine (PE, 10 µM). Activator of P2X receptors α,β-MeATP (100 µM) also relaxed segments precontracted with PE (10 µM). Activator of P2Y receptors UTP (1-100 µM) decreased and UTP (100-1000 µM) increased the amplitude of PE-induced contraction. Relaxing action of ATP was augmented by the donor of hydrogen sulfide NaHS (500 µM), but decreased in the presence of cystotionine-γ-lyase (CSE) DL-propargylglicine (10 mM). DL-propargylglicine (10 mM) increased the amplitude of constrictive action of UTP (100- 1000 µM), but decreased relaxing action of α,β-MeATP (100 µM). In conclusion, activation of P2X receptors decreases the amplitude of PE-induced contraction and activation of P2Y - increases it. Relaxing action of ATP in rat aorta segments with intact endothelium is mediated by CSE activation through P2X receptors.

 ТФ сероводород пуринергические рецепторы гладкомышечные клетки ATP hydrogen sulfide purinergic receptors smooth muscle cells

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