Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54317 Общая биофизика General biophysics Общая биофизика INVESTIGATING VISCOELASTIC PROPERTIES OF MAMMALIAN OOCYTES NUCLEOPLASM WITH OPTICAL TRAPPING ИССЛЕДОВАНИЕ ВЯЗКОУПРУГИХ СВОЙСТВ НУКЛЕОПЛАЗМЫ ООЦИТОВ МЛЕКОПИТАЮЩИХ С ПРИМЕНЕНИЕМ ОПТИЧЕСКОГО ЗАХВАТА Сырчина М С Syrchina M S wrongclue@gmail.com Залесский А Д Zalesskii A D Осыченко А А Osychenko A A Астафьев А А Astafiev A A Айбуш А В Aybush A V Надточенко В Н Nadtochenko V A Институт химической физики им. Н.Н. Семенова (ИХФ РАН) ru Semenov institute of chemical physics (ICP RAS) ru Институт химической физики им. Н.Н. Семенова (ИХФ РАН) ru Semenov institute of chemical physics (ICP RAS) ru Институт химической физики им. Н.Н. Семенова (ИХФ РАН) ru Semenov institute of chemical physics (ICP RAS) ru Институт химической физики им. Н.Н. Семенова (ИХФ РАН) ru Semenov institute of chemical physics (ICP RAS) ru Институт химической физики им. Н.Н. Семенова (ИХФ РАН) ru Semenov institute of chemical physics (ICP RAS) ru Институт химической физики им. Н.Н. Семенова (ИХФ РАН) ru Semenov institute of chemical physics (ICP RAS) ru 25 09 2018 25 09 2018 3 3 495 498 20 09 2018 20 09 2018 https://rusjbpc.ru/en/nauka/article/54317/view

В настоящем исследовании демонстрируется новый малоинвазивный метод изучения вязкоупругих свойств надмолекулярных комплексов клетки (хроматина, ядрышка) с применением оптического лазерного захвата. В качестве объекта исследования были выбраны ооциты мыши, в связи с тем, что их биохимия и внутриклеточная организация хорошо изучена, а ядро и ядрышко имеют крупные размеры (10 мкм). Ядрышко используется в качестве «живого» микрозонда, а с помощью оптического захвата вызываем возмущение в системе ядрышко-хроматин. Полученные времена релаксации ядрышка при его смещении оптическим пинцетом в разных направлениях указывают на анизотропные свойства нуклеоплазмы. Кроме того, использование оптического захвата на живых системах позволяет визуализировать взаимодействие ядерных и цитоплазматических структур, не прибегая к цитохимическим методам.

We suggest a new non-invasive method, based on applying of optical trapping for viscoelastic properties investigation of cellular supramolecular complexes (chromatin, nucleolus). As a model object, we used mice oocytes because of their well-known biochemistry, intracellular organization and large size of nuclei (10 μm). Nucleoli were supposed to be natural microspheres, so it was possible to catch them by optical tweezers and create deformations of nuclei matter. Based on our data, measured relaxation time of nucleoli could be an indicator of spatial viscoelastic properties changes. Also, optical trapping is a powerful tool for visualization of interactions between nucleus and cytoplasm.

 оптический захват ооцит мыши микрореология мягкие материалы ядрышко биополимеры optical trapping mouse oocyte microrheology soft matter nucleolus biopolymers

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