Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54436 Общая биофизика General biophysics Общая биофизика ANALYSIS OF THE AMINO-ACID SEQUENCE HOMOLOGY OF GLYCOSIDE-HYDROLASE FROM DIFFERENT PRODUCERS АНАЛИЗ СТЕПЕНИ ГОМОЛОГИИ АМИНОКИСЛОТНЫХ ПОСЛЕДОВАТЕЛЬНОСТЕЙ ГЛИКОЗИД-ГИДРОЛАЗ ИЗ РАЗЛИЧНЫХ ПРОДУЦЕНТОВ Сакибаев Ф А Sakibaev F A Макин С М Makin S M Холявка М Г Holyavka M G holyavka@rambler.ru Артюхов В Г Artyukhov V G Воронежский государственный университет ru Voronezh State University ru Воронежский государственный университет ru Voronezh State University ru Воронежский государственный университет ru Voronezh State University ru Воронежский государственный университет ru Voronezh State University ru 25 09 2019 25 09 2019 4 3 311 314 20 09 2019 20 09 2019 https://rusjbpc.ru/en/nauka/article/54436/view

Изучена степень гомологии аминокислотных последовательностей гликолитических ферментов: экзоинулиназ ADM21204.1, A.CAC44220.1, EHA22512.1, AHN08014.1, AGR40655.1 и BAC45010.1, эндоинулиназ AAN64131.1, ABB59681.1, EHA19510.1, XP_748286.1, ANY59682.1 и AA02437.1, β-фруктозидаз CAA04518.1 и CAA52620.1, фруктан-1-экзогидролазы CAC37922.1 и фруктозилтрансферазы ADK46938.1. Наибольшая степень идентичности аминокислотных последовательностей среди экзоинулиназ выявлена для ферментов из A. awamori и A. ficuum (90,88%), наименьшая - между энзимом из A. niger с ферментами из B. licheniformis и G. stearothermophilus (28,29%). Наибольшая степень идентичности для эндоинулиназ выявлена между двумя формами эндоинулиназы из A. niger (ABB59681.1 и EHA19510.1) и составляет 97,87%, что также является наибольшим показателем для всех представленных в работе ферментов, наименьшая - между ферментами из A. fumigatus и K. marxianus (27,89%). Наименьший показатель гомологичности среди всех представленных в работе ферментов выявлен между экзоинулиназой из A. niger и β-фруктозидазой из T. maritima и равен 20,45%. Приведенные в работе данные показывают значительную вариабельность аминокислотного состава карбогидраз, что указывает на широкие возможности их применения в различных условиях промышленного гидролиза инулина.

We studied the homology of amino acid sequences of glycolytic enzymes: exo-inulinases ADM21204.1, A.CAC44220.1, EHA22512.1, AHN08014.1, AGR40655.1 and BAC45010.1, endo-inulinases AAN64131.1, ABB59681.1, EHA19510.1, XP_748286 .1, ANY59682.1 and AA02437.1, β-fructosidases CAA04518.1 and CAA52620.1, fructan-1-exohydrolase CAC37922.1 and fructosyltransferase ADK46938.1. The highest amino acid sequences identity value among exo-inulinases was detected for the enzymes from A. awamori and A. ficuum (90.88%), the smallest is between the enzyme from A. niger with enzymes from B. licheniformis and G. stearothermophilus (28.29%). The highest identity value for endo-inulinases is detected between two forms of endo-inulinase from A. niger (ABB59681.1 and EHA19510.1) and amounts to 97.87%, which is also the highest indicator for all enzymes presented in the work, the smallest identity is between the enzymes from A. fumigatus and K. marxianus (27.89%). The smallest indicator of homology among all enzymes presented in the work was detected between exo-inulinase from A. niger and β-fructosidase from T. maritima and was equal to 20.45%. The data presented in the paper demonstrate a significant amino acid composition of carbohydrates, which indicates the wide possibilities of their use in various conditions of industrial hydrolysis of inulin.

 гликозид-гидролазы гомология аминокислотные последовательности glycoside-hydrolases homology amino acid sequences

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