Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54439 Общая биофизика General biophysics Общая биофизика EFFECT OF THE SURFACE COATING OF UPCONVERSION NANOPARTICLES ON THE COLLOIDAL STABILITY IN TRANSFERRIN SOLUTIONS ВЛИЯНИЕ ПОВЕРХНОСТНОГО ПОКРЫТИЯ АНТИСТОКСОВЫХ НАНОФОСФОРОВ НА КОЛЛОИДНУЮ СТАБИЛЬНОСТЬ В РАСТВОРАХ ТРАНСФЕРРИНА Воротнов А Д Vorotnov A D a.r.t.e.s@mail.ru Цареградская А И Tsaregradskaya A I Лианг Л Liang L Юдинцев А В Yudintsev A V Костюк А Б Kostyuk A B Звягин А. В. Zvyagin A. V. Национальный исследовательский Нижегородский государственный университет им. Н.И. Лобачевского ru Lobachevsky State University of Nizhni Novgorod ru Национальный исследовательский Нижегородский государственный университет им. Н.И. Лобачевского ru Lobachevsky State University of Nizhni Novgorod ru Университет Маккуори ru Macquarie University ru Национальный исследовательский Нижегородский государственный университет им. Н.И. Лобачевского ru Lobachevsky State University of Nizhni Novgorod ru Национальный исследовательский Нижегородский государственный университет им. Н.И. Лобачевского ru Lobachevsky State University of Nizhni Novgorod ru Национальный исследовательский Нижегородский государственный университет им. Н.И. Лобачевского Нижний Новгород Россия Lobachevsky State University of Nizhni Novgorod Nizhny Novgorod Russian Federation Первый МГМУ им. И.М. Сеченова Минздрава России (Сеченовский Университет) Москва Россия I.M. Sechenov First Moscow State Medical University Moscow Russian Federation Университет Маккуори Сидней Австралия Macquarie University Sydney Australia 25 09 2019 25 09 2019 4 3 327 334 20 09 2019 20 09 2019 https://rusjbpc.ru/en/nauka/article/54439/view

Коллоидная стабильность наночастиц (НЧ) играет ключевую роль в их успешном применении в различных биомедицинских приложениях. Насколько бы хорошо не были изучены их свойства в идеализированных условиях, все может кардинально измениться при взаимодействии с биологическими объектами. В живом организме НЧ в первую очередь встречают белки, которые формируют на их поверхности так называемую белковую корону. Хотя в большинстве случаев НЧ взаимодействуют со множеством белков одновременно, по-прежнему актуальными являются исследования взаимодействия НЧ с отдельными белками, а также последствий, к которым они приводят. В данной работе было исследовано влияние поверхностного покрытия наноразмерных антистоксовых фосфоров (НАФ) на коллоидную стабильность в растворах трансферрина (Тф). Показано, что в случае положительно заряженных НАФ-ПЭИ, Тф играет роль коагулянта и приводит к выпадению НЧ в осадок. В то время как коллоидная стабильность отрицательно заряженных НАФ-ПАК зависит от числа молекул Тф, приходящихся на одну НЧ. Так при избытке Тф в растворе наблюдается образование монослоя белка на поверхности НАФ-ПАК, который обеспечивает их коллоидную стабильность и может быть использован при разработке тераностических агентов, способных преодолеть гемато-энцефалический барьер.

The colloidal stability of nanoparticles (NPs) plays a key role in their successful usage in various biomedical applications. No matter how well their properties are studied under idealized conditions, everything can change dramatically while interacting with biological objects. In a living organism, NPs are primarily met by proteins, which form a so-called protein corona on their surface. Although in most cases NPs interact with many proteins simultaneously, studies of the interactions of NPs with individual proteins, as well as the consequences to which they lead, are still relevant. In this work, the effect of the surface coating of upconversion nanoparticles (UCNPs) on colloidal stability in transferrin (Tf) solutions was investigated. It is shown that in case of positively charged UCNP-PEI, Tf plays the role of a coagulant and leads to the precipitation of NPs. At the same time, the colloidal stability of negatively charged UCNP-PAA depends on the number of Tf molecules per one NP. So with an excess of Tf in the solution, a monolayer of protein is observed on the surface of UCNP-PAA, which ensures their colloid stability and can be used in the development of theranostics agents capable of overcoming the blood-brain barrier.

 белковая корона антистоксовые нанофосфоры трансферрин флуоресцентная корреляционная спектроскопия агрегация protein corona upconversion nanoparticles transferrin fluorescence correlation spectroscopy aggregation Работа выполнена при финансовой поддержке РФФИ (проект № 18-34-00723).

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