Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54448 Моделирование в биофизике Modelling in biophycis Моделирование в биофизике ECOLOGICAL BIOPHYSICS: MATHEMATICAL MODELLING EXPERIENSE OF TRANSGENIC MICROBIAL POPULATIONS DYNAMICS ЭКОЛОГИЧЕСКАЯ БИОФИЗИКА: ОПЫТ МАТЕМАТИЧЕСКОГО МОДЕЛИРОВАНИЯ ДИНАМИКИ ТРАНСГЕННЫХ МИКРОБНЫХ ПОПУЛЯЦИЙ Брильков А В Brilkov A V abrilkov@sfu-kras.ru Брилькова Е В Brilkova E V evmorbril@mail.ru Ганусов В В Ganusov V V Жабрун И В Jabrun I V Логинов Ю Ю Loginov Yu Yu loginov@sibsau.ru Сибирский федеральный университет ru Siberian Federal University ru Институт биофизики ФИЦ СО РАН ru Institute of biophysics FRC SB RAS ru Институт биофизики ФИЦ СО РАН ru Institute of biophysics FRC SB RAS ru Институт биофизики ФИЦ СО РАН ru Institute of biophysics FRC SB RAS ru Сибирский государственный университет науки и технологий им. ак. М.Ф. Решетнева ru Reshetnev Siberian State University of Science and Technology ru 25 09 2019 25 09 2019 4 3 378 382 20 09 2019 20 09 2019 https://rusjbpc.ru/en/nauka/article/54448/view

Настоящая статья посвящена математическому моделированию динамики популяций трансгенных микроорганизмов (ГМО) при культивировании в хемостате. Причины нестабильности трансгенных микроорганизмов при длительном периодическом или непрерывном культивировании в хемостате не совсем понятны до сих пор. Почему клетки, содержащие плазмиды с клонированными генами, растут медленнее бесплазмидных также неясно: или это прямое влияние экспрессии плазмидных генов на жизнеспособность плазмидсодержащих клеток, ингибирование клеточного роста или же расходование клеточных ресурсов на поддержание плазмид. Оценка параметров нестабильности плазмид с помощью методов, предложенных в настоящей работе, является первым шагом к пониманию главной причины: почему трансгенные микроорганизмы теряют плазмиды при культивировании и не теряют их в природе (плазмидный парадокс).

The paper is devoted to mathematical modeling of transgenic microbial populations (GMO) dynamics during cultivation in the chemostat. Even at present time we do not completely understand why and how transgenic microorganisms are unstable during prolonged butch or chemostat cultivation. The exact mechanisms by which containing cloned genes plasmid-bearing cells grow at a slower rate than their plasmid-free counterparts are also unclear whether it is a direct effect of plasmid gene expression on the viability of plasmid-bearing cells, inhibition of the cell growth, or the use of cell resources on plasmid maintenance. Estimation of the plasmid instability parameters according to the methods developed in this paper is the first step in understanding the major causes of why transgenic microorganisms plasmids are lost in culture or not lost in nature (plasmids paradox).

 трансгенные микроорганизмы нестабильность плазмид математическое моделирование стоимость плазмид непрерывное культивирование микроорганизмов хемостат transgenic microorganisms plasmids unstability mathematical modeling cost of plasmids continuous cultures of microorganisms chemostat

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