Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54478 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ ROLE OF METALLOTIONEINES IN MAINTAINS OF LEUKEMIC LYMPHOCYTES VIABILITY UNDER REDOX-BALANCE MODIFICATION УЧАСТИЕ МЕТАЛЛОТИОНЕИНОВ В ПОДДЕРЖАНИИ ЖИЗНЕСПОСОБНОСТИ ЛЕЙКОЗНЫХ ЛИМФОЦИТОВ ПРИ МОДИФИКАЦИИ ОКИСЛИТЕЛЬНО-ВОССТАНОВИТЕЛЬНОГО БАЛАНСА Тамашевский А В Tamashevski A V tayzoe@mail.ru Гармаза Ю М Harmaza Y M Белевич Е И Bialevich K I Слобожанина Е И Slobozhanina E I Институт биофизики и клеточной инженерии НАН Беларуси ru Institute of Biophysics and Cell Engineering of National Academy of Sciences of Belarus ru Институт биофизики и клеточной инженерии НАН Беларуси ru Institute of Biophysics and Cell Engineering of National Academy of Sciences of Belarus ru Институт биофизики и клеточной инженерии НАН Беларуси ru Institute of Biophysics and Cell Engineering of National Academy of Sciences of Belarus ru Институт биофизики и клеточной инженерии НАН Беларуси ru Institute of Biophysics and Cell Engineering of National Academy of Sciences of Belarus ru 25 12 2019 25 12 2019 4 4 558 563 20 12 2019 20 12 2019 https://rusjbpc.ru/en/nauka/article/54478/view

Проведена оценка уровня экспрессии цистеин-содержащих низкомолекулярных белков металлотионеинов и активности цистеиновой протеазы - каспазы-3 в лимфоцитах пациентов с хроническим В-лимфоцитарным лейкозом до и после модификации их редокс-статуса. При физиологических условиях в лимфоцитах пациентов с хроническим В-лимфоцитарным лейкозом обнаружена повышенная активность каспазы-3 по сравнению с нормальными клетками. H2O2-индуцированный окислительный стресс в лейкемических клетках приводил к запуску апоптотических процессов по каспазо-зависимому механизму. Установлено, что основным триггером в этом процессе могут выступать металлотионеины, как полипептиды, способные депонировать ионы цинка, и тем самым играть значительную роль в процессах, опосредованных цинковой сигнализацией, в лимфоцитах человека при хроническом В-лимфоцитарном лейкозе.

Expression of cysteine-containing low molecular weight proteins - metallothioneins and activity of cysteine protease - caspase-3 in lymphocytes of patients with chronic B-lymphocytic leukemia were evaluated before and after modification of their redox status. An increased caspase-3 activity was detected in lymphocytes of patients with B-CLL compared to normal cells under physiological condition. H2O2-induced oxidative stress in leukemic cells led to the activation of caspase-dependent apoptotic processes. It has been established that metallothioneins are the main triggers in this process. These polypeptides can deposit zinc ions and thereby play a significant role in process mediated by cell zinc signaling under chronic B-lymphocytic leukemia.

 хронический B-лимфоцитарный лейкоз металлотионеины каспаза-3 окислительно-восстановительный баланс цинковый гомеостаз chronic B-lymphocytic leukemia metallothioneins caspase-3 redox-state zinc homeostasis

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