Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54492 Общая биофизика General biophysics Общая биофизика Protein-protein interactions according to translational diffusion Белок-белковые взаимодействия по данным трансляционной диффузии Кусова А М Kusova A M alexakusova@mail.ru Ситницкий А Э Sitnitsky A E Зуев Ю Ф Zuev Yu F Казанский институт биохимии и биофизики ФИЦ КазНЦ РАН ru Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center, Russian Academy of Sciences ru Казанский институт биохимии и биофизики ФИЦ КазНЦ РАН ru Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center, Russian Academy of Sciences ru Казанский институт биохимии и биофизики ФИЦ КазНЦ РАН ru Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center, Russian Academy of Sciences ru 25 03 2020 25 03 2020 5 1 81 84 20 03 2020 20 03 2020 https://rusjbpc.ru/en/nauka/article/54492/view

В статье представлена информация о трансляционной диффузии неструктурированного αS-казеина и жесткого глобулярного α-химотрипсина в качестве инструмента для оценки межмолекулярных взаимодействий в водных белковых растворах. Коэффициенты самодиффузии и взаимной диффузии белков были получены методами Ядерного Магнитного Резонанса с Импульсным Градиентом Магнитного Поля (ЯМР ИГМП) и Динамического Рассеяния Света (ДРС). Теоретическое описание экспериментальных данных основывалось на фрикционном формализме неравновесной термодинамики. В результате были получены наборы вириальных коэффициентов, содержащие информацию о различных типах и вкладах межмолекулярных взаимодействий. Вторые вириальные коэффициенты были рассчитаны с помощью модели белок-белкового потенциала средней силы, которая включает в себя описание электростатических и дисперсионных взаимодействий. Предложенный комплексный подход позволил оценить вклады различных взаимодействий и описать межмолекулярные взаимодействия белков, принципиально отличающихся по форме и структуре.

The article provides information on translational diffusion of unstructured αS-casein and hard globular α-chymotrypsin as a tool for evaluating intermolecular interactions in aqueous protein solutions. The self- and mutual diffusion coefficients of proteins were obtained using Pulsed Field Gradient Nuclear Magnetic Resonance (PFG NMR) and Dynamic Light Scattering (DLS) methods. The theoretical description of the experimental data was based on the friction formalism of nonequilibrium thermodynamics [1]. As a result, sets of virial coefficients containing information on various types and contributions of intermolecular interactions were obtained. Also, the second virial coefficients were calculated from the model of the protein - protein potential of mean force, that contains description of charge - charge, charge - dipole, dipole - dipole potentials, dispersion Hamaker and the mean force osmotic-attraction potentials [2]. The proposed complex approach to the study of protein interactions made it possible to estimate the contribution of various interactions and described the intermolecular interactions of proteins that are fundamentally different in shape and structure.

 белок-белковые взаимодействия второй вириальный коэффициент трансляционная диффузия ЯМР ДРС protein-protein interactions second virial coefficient translational diffusion NMR PFG DLS Работа выполнена при поддержке Российского Фонда Фундаментальных Исследований, грант № 20-04-00157.

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