Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54523 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ Nutrition additive of E322 as phosphatidylcholine source for envelope in nanoscale formulation Nutrition additive of E322 as phosphatidylcholine source for envelope in nanoscale formulation Pogorelov A G Pogorelov A G agpogorelov@rambler.ru Stepanova T A Stepanova T A Pogorelova V N Pogorelova V N Suvorov O A Suvorov O A Gulin A A Gulin A A aleksandr.gulin@phystech.edu Institute of Theoretical and Experimental Biophysics RAS ru Institute of Theoretical and Experimental Biophysics RAS ru Institute of Theoretical and Experimental Biophysics RAS ru Institute of Theoretical and Experimental Biophysics RAS ru Institute of Theoretical and Experimental Biophysics RAS ru Institute of Theoretical and Experimental Biophysics RAS ru Institute of Theoretical and Experimental Biophysics RAS ru Institute of Theoretical and Experimental Biophysics RAS ru N.N. Semenov` Institute of Chemical Physics RAS ru N.N. Semenov` Institute of Chemical Physics RAS ru 25 06 2020 25 06 2020 5 2 286 290 20 06 2020 20 06 2020 https://rusjbpc.ru/en/nauka/article/54523/view

Microporous nanomaterials can provide interesting tools for different goals from bioimaging to the delivery of bioactive molecules. In this study, the procedure based on cryoapproaches was designed to formulate the nanoparticles of clinoptilolite from Zeolites mineral family. Applying scanning electron microscopy, clinoptilolite particles were imaged. Retting the nanoparticles in the ethanol solution of phosphatidylcholine (lecithin), the particle was encapsulated in lecitin envelope. Registering the diminution of optical density (OD 235) for ethanol/lecitin solution, the sorption of lecitin by clinoptilolite nanoparticles was studied with UV spectrometry. The kinetics of lecitin/clinoptilolite complex development was shown to exhibit intricate behavior, when the adsorption of lecitin was followed with its gradual desorption resulting in the increase of lecitin content in ethanol solution back toward the original level. The distribution of final size for lecitin/clinoptilolite complex was determined with dynamic light scattering technique. To our knowledge, there are no reports of natural zeolite nanoparticles that were used as the platform for nanosize capsule with phospholipids shell.

Microporous nanomaterials can provide interesting tools for different goals from bioimaging to the delivery of bioactive molecules. In this study, the procedure based on cryoapproaches was designed to formulate the nanoparticles of clinoptilolite from Zeolites mineral family. Applying scanning electron microscopy, clinoptilolite particles were imaged. Retting the nanoparticles in the ethanol solution of phosphatidylcholine (lecithin), the particle was encapsulated in lecitin envelope. Registering the diminution of optical density (OD 235) for ethanol/lecitin solution, the sorption of lecitin by clinoptilolite nanoparticles was studied with UV spectrometry. The kinetics of lecitin/clinoptilolite complex development was shown to exhibit intricate behavior, when the adsorption of lecitin was followed with its gradual desorption resulting in the increase of lecitin content in ethanol solution back toward the original level. The distribution of final size for lecitin/clinoptilolite complex was determined with dynamic light scattering technique. To our knowledge, there are no reports of natural zeolite nanoparticles that were used as the platform for nanosize capsule with phospholipids shell.

 phosphatidylcholine (lecithin) UV spectrometry scanning electron microscopy nanocapsule phosphatidylcholine (lecithin) UV spectrometry scanning electron microscopy nanocapsule This work was supported by the Russian Science Foundation grant № 20-16-00019.

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