Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54583 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ Role of the nitrogen oxide system and pro-inflammatory cytokines in the pathogenetic mechanisms of degenerative joint diseases Роль системы оксида азота и провоспалительных цитокинов в механизмах патогенеза дегенеративных заболеваний суставов Внуков В В Vnukov V V Кролевец И В Krolevets I V Плотников А А Plotnikov A A Ананян А А Ananyan A A Колесников М А Kolesnikov M A Милютина Н П Milyutina N P natmilut@rambler.ru Южный федеральный университет ru Southern Federal University, Academy of Biology and Biotechnology ru Ростовский государственный медицинский университет Минздрава РФ ru Rostov State Medical University ru Южный федеральный университет ru Southern Federal University, Academy of Biology and Biotechnology ru Южный федеральный университет ru Southern Federal University, Academy of Biology and Biotechnology ru Городская клиническая больница № 2 ru City Clinical Hospital No.2 ru Южный федеральный университет ru Southern Federal University, Academy of Biology and Biotechnology ru 25 12 2020 25 12 2020 5 4 665 671 20 12 2020 20 12 2020 https://rusjbpc.ru/en/nauka/article/54583/view

В работе исследовали уровни оксида азота, провоспалительных цитокинов (IL-1β, IL-6, IL-18, TNFα), лептина и мочевой кислоты в плазме крови и синовиальной жидкости (СЖ) как биомаркеров посттравматического гонартроза (ПТГА), а также их ассоциацию с рентгенологической стадией ПТГА. Установлено, что у пациентов с ранними и поздними стадиями посттравматического артроза коленного сустава повышен уровень циркулирующих провоспалительных медиаторов: нитритов/нитратов (NOx-), IL-6 и мочевой кислоты по сравнению со здоровыми лицами. Циркулирующие уровни NOx-, IL-1β, IL-6, мочевой кислоты, лептина и комбинации IL-6 и лептина могут быть потенциальными диагностическими тестами в соответствии с результатами ROC-анализа. Уровни NOx-, IL-6, IL-18 и лептина в плазме достоверно коррелировали с их содержанием в СЖ. Тяжесть ПTГA коррелировала с уровнем лептина в плазме и СЖ, а также с уровнем синовиального IL-18. Исследована ассоциация полиморфных вариантов генов nNOS и eNOS с риском развития посттравматического гонартроза (ПТГА) у жителей Ростовской области. Установлено, что аллель -84A полиморфного гена nNOS ассоциирован с риском развития ПТГА.

It was investigated the levels of nitric oxide, pro-inflammatory cytokines (IL-1β, IL-6, IL-18, TNFα), leptin and uric acid in blood plasma and synovial fluid (SF) as biomarkers of post-traumatic gonarthrosis (PTGA), as well as their association with radiological stage of PTGA. It was found that in patients with early and late stages of post-traumatic arthrosis of the knee joint, the level of circulating pro-inflammatory mediators: nitrites / nitrates (NOx), IL-6, and uric acid was increased compared with healthy individuals. Circulating levels of NOx, IL-1, IL-6, uric acid, leptin, and a combination of IL-6 and leptin can be potential diagnostic tests according to the ROC curve analysis. Plasma levels of NOx, IL-6, IL-18, and leptin significantly correlated with their levels in the SF. The severity of PTGA correlated with the level of leptin in plasma and SF, as well as with the level of synovial IL-18. The association of polymorphic variants of the nNOS and eNOS genes with the risk of developing post-traumatic gonarthrosis (PTGA) in residents of the Rostov region was studied. It has been established that the -84A allele of the nNOS polymorphic gene is associated with the risk of developing PTGA.

 посттравматический гонартроз плазма крови синовиальная жидкость оксид азота провоспалительные цитокины лептин гены NO-синтаз post-traumatic gonarthrosis blood plasma synovial fluid nitric oxide pro-inflammatory cytokines leptin NO synthase genes

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