Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54584 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ Expression of transport and cytosolic proteins under the formation of multidrug resistance in human myeloma cells in vitro Экспрессия транспортных и цитозольных белков при формировании множественной лекарственной устойчивости в клетках миеломы человека in vitro Тамашевский А В Tamashevski A V tayzoe@mail.ru Гармаза Ю М Harmaza Y M Пасюков В В Pasiukov V V Слобожанина Е И Slobozhanina E I Институт биофизики и клеточной инженерии НАН Беларуси ru Institute of Biophysics and Cell Engineering of National Academy of Sciences of Belarus ru Институт биофизики и клеточной инженерии НАН Беларуси ru Institute of Biophysics and Cell Engineering of National Academy of Sciences of Belarus ru Республиканский научно-практический центр трансфузиологии и медицинских биотехнологий МЗ РБ ru Republican Scientific and Practical Center for Transfusiology and Medical Biotechnologies MH RB ru Институт биофизики и клеточной инженерии НАН Беларуси ru Institute of Biophysics and Cell Engineering of National Academy of Sciences of Belarus ru 25 12 2020 25 12 2020 5 4 672 679 20 12 2020 20 12 2020 https://rusjbpc.ru/en/nauka/article/54584/view

Проведена оценка содержания транспортных белков, ассоциированных с феноменом множественной лекарственной устойчивости (P-гликопротеина и белка множественной лекарственной резистентности MRP1) и уровня экспрессии цистеин-содержащих низкомолекулярных белков металлотионеинов в лимфоцитах условно здоровых доноров, B-лимфобластоидной клеточной линии IM9 и ее сублинии, резистентной к флударабелу. Обнаружено снижение соотношения экспрессии P-гликопротеина к MRP1 в B-лимфобластоидной клеточной сублинии IM9, резистентной к флударабелу, относительно ее родительской линии IM9. Установлено, что одним из механизмов, вносящих вклад в развитие множественной лекарственной устойчивости в клеточной линии IM9 наряду с возрастанием роли белка MRP1 также выступает активация цистеин-обогащенных цитозольных белков металлотионеинов I/II классов.

The estimation of transport proteins associated with the phenomenon of multidrug resistance (P-glycoprotein and the protein of multidrug resistance - MRP1) amount and the cysteine-containing low-molecular-weight proteins metallothioneins level of expression in donor’s lymphocytes, B-lymphoblastoid cell line IM9 and its subline resistant to fludarabel were conducted. It was found decrease in the ratio of expression of P-glycoprotein to MRP1 in IM9 subline resistant to fludarabel relative to IM9 parental cell line. So, one of the mechanisms contributing to the development of multidrug resistance in the IM9 cell line, equally with increase of MRP1 protein importance, take place cysteine-enriched cytosolic proteins metallothioneins I/II classes activation as was found.

 клеточная линия IM9 белки множественной лекарственной устойчивости P-гликопротеин и MRP1 металлотионеины противоопухолевые препараты cell line IM9 multidrug resistance proteins P-glycoprotein and MRP1 metallothioneins anticancer drugs

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