Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54608 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ Study of the technology of the recombinate target protein selective labeling and development of the synthetic routes for the modified CdTe quantum dots synthesis in aqueous solutions Исследование технологии селективного маркирования рекомбинатных белков-мишеней и разработка путей синтеза модифицированных квантовых точек CdTe в водных растворах Беликов Н Е Belikov N E Демина О В Demina O V Лукин А Ю Lukin A Yu Петровская Л Е Petrovskaya L E Складнев Д А Skladnev D A Варфоломеев С Д Varfolomeev S D Ходонов А А Khodonov A A khodonov@gmail.com Институт биохимической физики им. Н.М. Эмануэля РАН ru N.M. Emanuel Institute of Biochemical Physics, RAS ru Институт биохимической физики им. Н.М. Эмануэля РАН ru N.M. Emanuel Institute of Biochemical Physics, RAS ru МИРЕА - Российский технологический университет ru MIREA - Russian Technological University ru Институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова РАН ru M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, RAS ru ФИЦ «Фундаментальные основы биотехнологии» РАН ru Federal Research Centre «Fundamentals of Biotechnology», RAS ru Институт биохимической физики им. Н.М. Эмануэля РАН ru N.M. Emanuel Institute of Biochemical Physics, RAS ru Институт биохимической физики им. Н.М. Эмануэля РАН ru N.M. Emanuel Institute of Biochemical Physics, RAS ru 25 03 2021 25 03 2021 6 1 88 96 20 03 2021 20 03 2021 https://rusjbpc.ru/en/nauka/article/54608/view

В работе рассматривается предложенная авторами процедура обратимого маркирования белков-мишеней с помощью флуоресцентных меток и зондов. В качестве последних было предложено использовать новые флуорофоры на основе квантовых точек (QDs CdTe), содержащие молекулярный адрес (хелатные комплексы Ni2+ с нитрилотриуксусной кислотой и ее производными), способные образовывать прочные хелаты с гексагистидиновым тегом, локализованном в заданном месте полипептидной цепи белка-мишени. В настоящей работе особое внимание посвящено выбору нужного состава и строения молекулы метки и реализации на практике лабораторной технологии синтеза набора исходных квантовых точек QDs CdTe с заданными оптическими параметрами.

This paper considers the proposed procedure for reversible labeling of target proteins using fluorescent labels and probes. New fluorophores based on quantum dots (QDs CdTe) containing a molecular address (chelate complexes of Ni2+ with nitrilotriacetic acid and its derivatives) were proposed, capable of forming strong chelates with a hexahistidine tag localized at a given site of the polypeptide chain of the target protein. In this work, special attention is devoted to the selection of the required composition and structure of the label molecule and the practical implementation of the laboratory technology for the synthesis of a set of initial QDs CdTe quantum dots samples with specified optical parameters.

 селективное маркирование белков-мишеней флуоресцентные метки квантовые точки методы получения нитрилотриуксусная кислота selective labeling of target proteins fluorescent labels quantum dots production methods nitrilotriacetic acid Исследование выполнено при финансовой поддержке РФФИ в рамках научного проекта № 20-03-00139.

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