Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54671 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ Hydrogen sulfide mediates NO-signaling activity, thereby stimulating biofilm dispersion Сероводород выступает медиатором NO-сигнальной активности, тем самым стимулируя дисперсию биопленок Телегина Д И Telegina D I daryakinder62@gmail.com Васильева С. В. Vasilyeva S. V. svvs0709@mail.ru Российский химико-технологический университет имени Д.И. Менделеева; Первый Московский государственный медицинский университет им. И. М. Сеченова ru D. Mendeleev University of Chemical Technology of Russia; I.M. Sechenov First Moscow State Medical University ru Институт биохимической физики им. Н.М. Эмануэля РАН Москва Россия N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences Moscow Russian Federation 25 09 2021 25 09 2021 6 3 439 446 20 09 2021 20 09 2021 https://rusjbpc.ru/en/nauka/article/54671/view

Впервые был применен комплексный подход к изучению взаимодействия монооксида азота и сероводорода-газотрансмиттеров, которые продуцируются бактериальными клетками в качестве сигнальных молекул. При исследовании кристаллических нитрозильных комплексов железа было установлено, что комбинированная обработка NO-донором и сероводородом, при условии 2.5-кратного избытка H2S на каждую NO-группу донора, стимулирует увеличение NO-сигнальной активности за счет образования более активных ДНКЖ с персульфидными лигандами в клетке, которые, в отличие от ДНКЖ с тиоловыми лигандами, дают характерный «узкий» ЭПР сигнал с gaver = 2,03 (g⊥ = 2.032, g|| = 2,02). Таким образом, H2S выступает медиатором NO-сигнальной активности, тем самым стимулируя значительное увеличение уровня экспрессии гена soxS. Влияние комбинированной обработки на уровень экспрессии гена sfiA обнаружено не было. Данное явление обуславливает снижение уровня окислительного стресса при совместной обработке NO-донором и H2S, что оказывает значительное влияние на снижение продуктивности формирования биопленок (в среднем на 30% относительно монообработки) и увеличение показателей дисперсии зрелых биопленок (в среднем на 25% относительно монообработки). Мы предполагаем, что применение комплексной обработки кристаллическими нитрозильными комплексами железа с сероводородом может быть востребовано для решения проблем борьбы с инфекциями, вызываемыми бактериальными биопленками.

In this study we first took an integrated approach to the interaction of NO and H2S gases, produced by the bacterial cells within their signaling potencies in DNA repair responses to oxidative damages and control of bacterial biofilm production. In the study of crystalline nitrosyl iron complexes, it was found that combined treatment with an NO donor and hydrogen sulfide, under the condition of a 2.5-fold excess of H2S for each NO-donor group, stimulates an increase in NO-signaling activity due to the formation of more active DNICs with persulfide ligands in the cell, which, in contrast to DNIC with thiol ligands, give a characteristic “narrow” EPR signal with gaver = 2.03 (g⊥ = 2.032, g || = 2.02). Thereby, H2S acts as a mediator of NO signaling activity, thereby stimulating a significant increase in the level of soxS gene expression. The effect of the combined treatment on the level of sfiA gene expression was not found. This phenomenon causes a decrease in the level of oxidative stress during joint treatment with a NO donor and H2S, which has a significant effect on a decrease in the productivity of biofilm formation (on average by 30% relative to mono-treatment) and an increase in the dispersion indicators of mature biofilms (on average by 25% relative to mono-treatment). We suppose that our findings with the complex NO-donors with H2S application may be useful in solving the bacterial biofilm problems.

 монооксид азота сероводород биопленки сигнальная активность nitric oxide hydrogen sulfide biofilm signaling functions

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