Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 54713 ЭКОЛОГИЧЕСКАЯ БИОФИЗИКА ECOLOGICAL BIOPHYSICS ЭКОЛОГИЧЕСКАЯ БИОФИЗИКА Assignment of functions to opsins of Trichoplax Adhaerens and Trichoplax sp. H2 Присваивание функций опсинам трихоплаксов Trichoplax Adhaerens и Trichoplax sp. H2 Хавронюк И. С. Khavronyuk I. S. havronuk.1994@gmail.com Мамонтов А. А. Mamontov A. A. Булков В А Bulkov V A Воронин Д П Voronin D P Кузнецов А В Kuznetsov A V Севастопольский государственный университет Севастополь Россия Sevastopol State University Sevastopol Russian Federation Севастопольский государственный университет Севастополь Россия Sevastopol State University Sevastopol Russian Federation Севастопольский государственный университет ru Sevastopol State University ru Севастопольский государственный университет ru Sevastopol State University ru ФИЦ «Институт биологии южных морей имени А.О. Ковалевского РАН» ru A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS ru 25 12 2021 25 12 2021 6 4 686 694 20 12 2021 20 12 2021 https://rusjbpc.ru/en/nauka/article/54713/view

В геноме трихоплакса Trichoplax sp. H2 присутствует 10 генов, кодирующих светочувствительные белки-опсины, из них 7 аннотированы как цветочувствительные опсины, от зелёного до ультрафиолетового. В свою очередь, в геноме трихоплакса Trichoplax adhaerens также обнаружены 10 опсино-подобных генов. Для половины из этих полипептидов восстановлены полные аминокислотные последовательности. В силу того, что опсины Trichoplax adhaerens (гаплотип H1) охарактеризованы неполно, представляло интерес провести дополнительное исследование опсинов трихоплакса гаплотипа H1. Для этого были осуществлены множественные выравнивания 20 аминокислотных последовательностей опсинов обоих штаммов трихоплакса и построены деревья родства опсинов. С помощью гомологического сравнения были созданы структурные модели исследуемых протеинов и осуществлён докинг хромофора ретиналя. На основании проведённых расчётов 3 полным гипотетическим белкам: EDV19328.1 длиной 465 aa, EDV22726.1 длиной 365 aa и EDV22897.1 длиной 319 aa из Trichoplax adhaerens была присвоена функция чувствительности к голубому свету. С меньшим правдоподобием чувствительность к голубому свету может быть присвоена 4 неполным гомологичным белкам: EDV22727.1 длиной 287 aa, EDV19349.1 - 188 aa, EDV19891.1 - 161 aa и EDV18601.1 длиной 137 aa из штамма H1. Присутствие множественных генов опсинов в геномах трихоплаксов гаплотипов H1 и H2 позволяет предположить, что Placozoa способны различать оттенки голубого цвета, а это, возможно, позволяет им ориентироваться в тоще морской воды.

The genome of Trichoplax sp. H2 has 10 genes encoding opsin, of which 7 ones are annotated as color-sensitive opsins, from green to ultraviolet. In turn, 10 opsin-like genes were also found in the genome of Trichoplax adhaerens . Full amino acid sequences were inferred for a half of these proteins. Due to the opsins of Trichoplax adhaerens haplotype H1 have been incompletely characterized, it was of special interest to investigate those opsins. For this purpose, multiple sequence alignments of the all 20 opsins from both trichoplax strains were performed, and guide trees were constructed. Structural models of the 20 proteins were created using homologous comparison and then the docking of retinal chromophore was performed. Based on these calculations, 3 complete hypothetical proteins, such as EDV19328.1 of length 465 aa, EDV22726.1 of length 365 aa, and EDV22897.1 of length 319 aa from Trichoplax adhaerens were assigned a blue light sensitivity function. With less chance, the blue light sensitivity could be assigned to 4 incomplete homologous proteins, such as EDV22727.1 (287 aa), EDV19349.1 (188 aa), EDV19891.1 (161 aa), and EDV18601.1 (137 aa) from strain H1. The being of multiple opsin genes in the genomes of haplotypes H1 and H2 suggests that Placozoa are capable of distinguishing shades of blue, and this possibly allows them to navigate in the seawater.

 аннотация биологические функции гомология структурное моделирование опсины Placozoa annotation biological functions homology structural modeling opsins Placozoa

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