Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 55005 10.29039/rusjbpc.2022.0506 Моделирование в биофизике Modelling in biophycis Моделирование в биофизике OPSINS OF THE CTENOPHORE MNEMIOPSIS LEIDYI AND A NETWORK OF PROTEIN-PROTEIN INTERACTIONS Опсины гребневика Mnemiopsis leidyi и сеть белок-белковых взаимодействий Сергеева Е. В. Sergeeva E. V. Фадеева М. В. Fadeeva M. V. Хавронюк И. С. Khavronyuk I. S. havronuk.1994@gmail.com Мамонтов А. А. Mamontov A. A. Ершов А. Б. Ershov A. B. Кузнецов А. В. Kuznetsov A. V. kuznet61@gmail.com Севастопольский государственный университет Севастополь Россия Sevastopol State University Sevastopol Russian Federation Севастопольский государственный университет Севастополь Россия Sevastopol State University Sevastopol Russian Federation Севастопольский государственный университет Севастополь Россия Sevastopol State University Sevastopol Russian Federation Севастопольский государственный университет Севастополь Россия Sevastopol State University Sevastopol Russian Federation Институт биологии южных морей им. А.О. Ковалевского РАН Севастополь Россия A.O. Kovalevsky Institute of Biology of the South Seas of the RAS Sevastopol Russian Federation Центр дополнительного образования «Малая академия наук» Севастополь Россия Center for Additional Education "Small Academy of Sciences" Sevastopol Russian Federation Институт биологии южных морей им. А.О. Ковалевского РАН Севастополь Россия A.O. Kovalevsky Institute of Biology of the South Seas of the RAS Sevastopol Russian Federation Севастопольский государственный университет Севастополь Россия Sevastopol State University Sevastopol Russian Federation 25 06 2022 25 06 2022 7 2 222 229 20 06 2022 20 06 2022 https://rusjbpc.ru/en/nauka/article/55005/view

Представители типа Ctenophora являются древней и обособленной ветвью эволюционного древа развития органического мира, обитают большей частью в эпипелагиали морей и демонстрируют удивительные механизмы приспособления к окружающей среде. С развитием техники секвенирования ДНК появилась возможность исследовать биологические объекты не только экспериментально, но и теоретически. С помощью методов биоинформатики изучали опсины гребневика Mnemiopsis leidyi A. Agassiz 1865. Проведена дополнительная аннотация опсинов 1, 2 и 3, AFK83788.1, AFK83789.1 и AFK83790.1, соответственно. Построены их 3D-модели и определена локализация ретиналя внутри молекул белков. Исследовано белковое окружение опсинов, охарактеризованы соседи-протеины первого и второго уровня. Показано, что структура сети белок-белковых взаимодействий напоминает перцептрон, а, следовательно, может выполнять функцию распознавания паттернов поступающих извне сигналов. Выявлена многозадачность проводящих путей от эндоцитоза до Wnt-сигнализации, что указывает на участие опсинов гребневиков в широком спектре генетических и физиологических процессов.

Ctenophora are ancient and separate branch of evolutionary tree of the organic world development. They inhabit mostly epipelagic seas and demonstrate amazing mechanisms of adaptation to the environment. It is now possible with the potential technique of DNA sequencing to study biological objects not only experimentally but also theoretically. We studied opsins of the ctenophore Mnemiopsis leidyi A. Agassiz 1865 using bioinformatics methods. Additional annotation of opsins 1, 2, and 3, AFK83788.1, AFK83789.1, and AFK83790.1, respectively, was performed. Their 3D models were constructed and the localization of retinal within the protein molecules was determined. The protein environment of opsins was studied, and the first- and second-level protein neighbors were characterized. The structure of the protein-protein interaction network was shown to resemble a perceptron and, therefore, to perform the function of recognizing the patterns of the signals coming from outside. The multitasking of signaling pathways from endocytosis to Wnt signaling has been revealed, indicating the involvement of Ctenophora opsins in a wide range of genetic and physiological processes.

 Ctenophora опсины сигнальная трансдукция Ctenophora opsins signal transduction Работа выполнена в рамках государственного задания ФИЦ ИнБЮМ тема № 0828-2018-0002 и проекта «Трихоплакс для бионики II» программы Сириус.Лето: начни свой проект. Сезон 2021/2022 № 100220210118094093.

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