Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 55016 10.29039/rusjbpc.2022.0516 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ EVALUATION OF THE HUMAN BLOOD PLATELETS SENSITIVITY TO AN ACTIVATION AGONIST ADENOSINE DIPHOSPHATE BY SCANNING FLOW CYTOMETRY UNDER CONDITIONS OF ANTIPLATELET THERAPY Оценка чувствительности тромбоцитов крови человека к агонисту активации аденозиндифосфату методом сканирующей проточной цитометрии в условиях применения антитромбоцитарной терапии Литвиненко А. Л. Litvinenko A. L. roseline.neolis@gmail.com Некрасов В. М. Nekrasov V. M. Четвертак Е. В. Chetvertak E. V. Мальцев В. П. Maltsev V. P. Институт химической кинетики и горения им. В.В. Воеводского Новосибирск Россия Voevodsky Institute of Chemical Kinetics and Combustion Novosibirsk Russian Federation Институт химической кинетики и горения им. В.В. Воеводского Новосибирск Россия Voevodsky Institute of Chemical Kinetics and Combustion Novosibirsk Russian Federation Институт химической кинетики и горения им. В.В. Воеводского Новосибирск Россия Voevodsky Institute of Chemical Kinetics and Combustion Novosibirsk Russian Federation Институт химической кинетики и горения им. В.В. Воеводского Новосибирск Россия Voevodsky Institute of Chemical Kinetics and Combustion Novosibirsk Russian Federation 25 06 2022 25 06 2022 7 2 286 292 20 06 2022 20 06 2022 https://rusjbpc.ru/en/nauka/article/55016/view

Сердечно-сосудистые заболевания занимают лидирующее место среди причин смертности населения. Основные осложнения течения заболевания этой группы тесно связаны с изменением в работе системы гемостаза, в том числе её тромбоцитарного звена. Для предотвращения развития осложнений, связанных с нарушениями в работе системы гемостаза, применяется антитромбоцитарная терапия. В данной работе рассматривается поведение популяции тромбоцитов условно здоровых доноров до и после применения двух основных типов терапевтических агентов: ацетилсалициловой кислоты и клопидогрела. С использованием метода сканирующей проточной цитометрии производилось измерение индекса формы одиночных тромбоцитов с последующем построением распределений по данной характеристике для проб в нативном состоянии и после воздействия малого количества аденозиндифосфата - агониста активации. По изменению в распределениях индекса формы оценивается чувствительность тромбоцитов к агонисту активации до и после применения краткого курса терапии. По результату оценки пороговое значение начала изменения формы тромбоцитов и чувствительность при применении ацетилсалициловой кислоты увеличились, а при применении клопидогрела уменьшились. Продемонстрированный подход для оценки чувствительности может быть полезен как при оценке степени эффективности, применяемой антитромбоцитарной терапии, так и при диагностике наличия осложнений течения заболеваний сердечно-сосудистой системы со стороны тромбоцитарного звена системы гемостаза.

Cardiovascular diseases occupy a leading place among the causes of death in the population. The main complications of the disease in this group are closely related to changes in the functioning of the hemostasis system, including primary hemostasis. Antiplatelet therapy is used to prevent the development of complications associated with disorders in the hemostasis system. This paper examines the behavior of the platelet population of healthy donors before and after the use of two main types of drugs: acetylsalicylic acid and clopidogrel. Using the method of scanning flow cytometry, the shape index of single platelets was measured, followed by the construction of distributions according to this characteristic for native samples and after exposure to a small amount of an activation agonist. The sensitivity of platelets to the activation agonist is assessed by the change in distributions before and after the application of a short course of therapy. As a result of the assessment, the threshold value for the onset of changes in the shape of platelets and sensitivity increased with the use of acetylsalicylic acid, and decreased with the use of clopidogrel. The demonstrated approach for assessing sensitivity can be useful both in assessing the degree of effectiveness of antiplatelet therapy used and in diagnosing the presence of complications in the course of diseases of the cardiovascular system on the part of primary hemostasis.

 тромбоциты сканирующая проточная цитометрия антитромбоцитарная терапия АДФ platelets scanning flow cytometry antiplatelet therapy ADP Работа выполнена при финансовой поддержке в форме стипендии Президента Российской Федерации для аспирантов и молодых учёных.

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