Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 83236 10.29039/rusjbpc.2023.0607 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ NITRIC OXIDE IS A HIGHLY EFFICIENT ROS TRAP. POSSIBILITY OF CLINICAL USE FOR DIAGNOSTICS ОКСИД АЗОТА – ВЫСОКОЭФФЕКТИВНАЯ ЛОВУШКА АФК. ВОЗМОЖНОСТИ КЛИНИЧЕСКОГО ИСПОЛЬЗОВАНИЯ ДЛЯ ДИАГНОСТИКИ Титов В. Ю. Titov V. Yu. vtitov43@yandex.ru Осипов А. Н. Osipov A. N. Ананкина А. А. Anankina A. A. Кочиш И. И. Kochish I. I. kochish.i@mail.ru Российский национальный исследовательский медицинский университет имени Н.И. Пирогова Москва Россия Pirogov Russian National Research Medical University Moscow Russian Federation Московская государственная академия ветеринарной медицины и биотехнологии – МВА имени К.И. Скрябина Москва Россия Moscow State Academy of Veterinary Medicine and Biotechnology - MVA by K.I. Skryabin Moscow Russian Federation ФНЦ Всероссийский научно-исследовательский и технологический институт птицеводства РАН Сергиев Посад Россия Russian National Research and Technological Poultry Farming Institute, RAS Sergiev Posad Russian Federation Российский национальный исследовательский медицинский университет имени Н.И. Пирогова Москва Россия Pirogov Russian National Research Medical University Moscow Russian Federation Российский национальный исследовательский медицинский университет им. Н.И. Пирогова Минздрава России Москва Россия Pirogov Russian National Research Medical University Moscow Russian Federation Московская государственная академия ветеринарной медицины и биотехнологии - МВА имени К.И. Скрябина Россия Moscow state Academy of Veterinary Medicine and Biotechnology - MVA by K.I. Skryabin Russian Federation 21 05 2024 21 05 2024 8 2 185 193 15 07 2023 https://rusjbpc.ru/en/nauka/article/83236/view

Показано, что в норме нитрит присутствует в большинстве тканей в концентрации, не превышающей 50 нМ. Но ткани содержат десятки микромоль соединений – доноров NO. Следовательно, в тканях есть механизмы, предотвращающие окисление NO до нитрита. Соединения-доноры NO спонтанно не распадаются с высвобождением NO. Трансформация NO, включенного в состав соединений доноров, до нитрита и нетиолатных нитрозосоединений (NO2- + RNO) происходит под действием активных форм кислорода (АФК) и, прежде всего, супероксида (О2-). Последний продуцируется активированными фагоцитами. Таким образом, содержание NO2- + RNO – очень чувствительный показатель активации фагоцитов – процесса, сопровождающего любое воспаление. В данной работе рассмотрена возможность использования показателя содержания NO2- + RNO для ранней диагностики заболеваний, носящих воспалительный характер. Показано, что этот показатель обладает большей чувствительностью и специфичностью, чем все известные клинические и биохимические показатели. Это делает его особенно ценным для контроля состояния больных в стационаре, контроля состояния сельскохозяйственных животных. Фактором, ограничивающим использование показателя NO2- + RNO в диагностике является проницаемость тканевых барьеров для этих соединений. Это особенно актуально для контроля состояния плода, а также состояния центральной нервной системы.

It has been shown that nitrite is normally present in most tissues at a concentration not exceeding 50 nM. But the tissues contain NO donor compounds in concentration dozens of micromoles. Consequently, there are mechanisms in the tissues that prevent the oxidation of NO to nitrite. The NO donor compounds do not spontaneously dissociate with the release of NO. The transformation of NO included in the composition of donor compounds to nitrite and non-thiolate nitroso compounds (NO2- + RNO) occurs under the action of active oxygen species (ROS) and, above all, superoxide that is produced by activated phagocytes. Thus, the content of NO2- + RNO is a very sensitive indicator of phagocyte activation, a process that accompanies any inflammation. In this paper, the possibility of using the NO2- + RNO content as indicator for the early diagnosis of inflammatory diseases is considered. It has been shown that this indicator has greater sensitivity and specificity than all known clinical and biochemical indicators. This makes it especially valuable for monitoring the state of patients in the hospital, monitoring the farm animals. The factor limiting the use of the NO2- + RNO indicator in diagnostics is the permeability of tissue barriers to these compounds. This is especially important for monitoring the condition of the fetus, as well as the state of the central nervous system.

 оксид азота (NO) нитрит и нетиолатные нитрозосоединения (NO2- + RNO) фагоциты воспаление nitric oxide (NO) nitrite and non-thiolate nitroso compounds (NO2- + RNO) phagocytes inflammation

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