Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 83372 10.29039/rusjbpc.2023.0629 МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY МЕДИЦИНСКАЯ БИОФИЗИКА И БИОФИЗИЧЕСКАЯ ХИМИЯ FEATURES OF PHOTOBIOMODULATION DURING ONCOGENESIS DEPENDING ON THE INTENSITY OF OXIDATIVE PROCESSES IN THE ORGANISM OF RATS ОСОБЕННОСТИ ФОТОБИОМОДУЛЯЦИИ ПРИ ОНКОГЕНЕЗЕ В ЗАВИСИМОСТИ ОТ ИНТЕНСИВНОСТИ ОКИСЛИТЕЛЬНЫХ ПРОЦЕССОВ В ОРГАНИЗМЕ КРЫС Позднякова М. А. Pozdnyakova M. A. Жукова Е. С. Zhukova E. S. evgenya_plekhanova@mail.ru Нижегородский научно-исследовательский институт гигиены и профпатологии Роспотребнадзора Нижний Новгород Россия Nizhny Novgorod Scientific Research Institute for Hygiene and Occupational Pathology Nizhny Novgorod Russian Federation Нижегородский научно-исследовательский институт гигиены и профпатологии Роспотребнадзора Нижний Новгород Россия Nizhny Novgorod Scientific Research Institute for Hygiene and Occupational Pathology Nizhny Novgorod Russian Federation 27 05 2024 27 05 2024 8 3 333 336 21 07 2023 https://rusjbpc.ru/en/nauka/article/83372/view

В работе проверяется гипотеза о зависимости противоопухолевой эффективности фотобиомодуляции (ФБМ) от интенсивности окислительной модификации макромолекул. Об уровне окислительных процессов в организме судили по содержанию в биологических тканях и жидкостях, индуцированных активными формами кислорода (АФК) продуктов модификации липидов, белков, ДНК, а также активности антиоксидантных ферментов супероксиддисмутазы (СОД) и каталазы. Предлагается использовать поведенческую активность животных в тесте «открытое поле» как неинвазивный показатель для прогнозирования биологических эффектов фотобиомодуляции, косвенно отражающий степень окислительной напряженности в организме. Показано, что уровень поведенческой активности коррелирует с активностью СОД в печени (ρ=0,8, p <0,03 по критерию Спирмена). Среди лабораторных крыс можно выделить животных с пассивным, среднеактивным и высоактивным типом поведения. Для каждого типа характерны различная выраженность окислительных процессов, динамика роста модельных солидных опухолей и ответ на ФБМ фиолетово-синей и красной области спектра. Внутрибрюшинное воздействие озонированным физиологическим раствором с концентрацией озона 0,16 мкг/кг модулирует противоопухолевый эффект ФБМ. Делается вывод, что эффективность действия ФМБ при онкогенезе зависит от интенсивности окислительных процессов в организме и взаимосвязана с особенностями поведения крыс.

In work tested the hypothesis that the antitumor efficacy of photobiomodulation (PBM) depends on the intensity of oxidative modification of macromolecules. The level of oxidative processes in the body was judged by the content of ROS-induced products of lipid, protein, and DNA modification, as well as the activity of the antioxidant enzymes superoxide dismutase (SOD) and catalase in biological tissues and fluids. It is proposed to use the behavioral activity of animals in the "open field" test as a non-invasive indicator for predicting the biological effects of photobiomodulation, indirectly reflecting the degree of oxidative stress in the body. It was shown that the level of behavioral activity correlates with SOD activity in the liver (ρ=0.8, p < 0.03, Spearman's test). Among laboratory rats, animals with passive, moderately active and highly active types of behavior can be distinguished. Each type is characterized by different severity of oxidative processes, growth dynamics of model solid tumors, and response to PBM in the violet-blue and red regions of the spectrum. Intraperitoneal exposure to ozonized saline with an ozone concentration of 0.16 μg/kg modulates the antitumor effect of PBM. It is concluded that the effectiveness of PMB during oncogenesis depends on the intensity of oxidative processes in the body and is interrelated with the behavioral characteristics of rats.

 фотобиомодуляция солидные модели опухолевого роста перекисное окисление липидов окислительная модификация белков уровень повреждений ДНК активность антиоксидантных ферментов поведенческая активность тест «открытое поле» photobiomodulation solid models of tumor growth lipid peroxidation oxidative modification of proteins level of DNA damage activity of antioxidant enzymes behavioral activity open field test

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