Russian Journal of Biological Physics and Chemisrty Russian Journal of Biological Physics and Chemisrty АКТУАЛЬНЫЕ ВОПРОСЫ БИОЛОГИЧЕСКОЙ ФИЗИКИ И ХИМИИ 2499-9962 83718 10.29039/rusjbpc.2023.0652 БИОФИЗИЧЕСКАЯ ЭКОЛОГИЯ И БИОЛОГИЧЕСКИЕ РЕСУРСЫ BIOPHYSICAL ECOLOGY AND BIOLOGICAL RESOURCES БИОФИЗИЧЕСКАЯ ЭКОЛОГИЯ И БИОЛОГИЧЕСКИЕ РЕСУРСЫ THE EFFECT OF SHORT-TERM SALINITY CHANGES ON THE FUNCTIONAL FEATURES OF MEDITERRANEAN MUSSEL HEMOCYTES ВЛИЯНИЕ КРАТКОСРОЧНОГО ИЗМЕНЕНИЯ СОЛЕНОСТИ НА ФУНКЦИОНАЛЬНЫЕ ОСОБЕННОСТИ ГЕМОЦИТОВ СРЕДИЗЕМНОМОРСКОЙ МИДИИ Лавриченко Д. С. Lavrichenko D. S. dlavrichenko01@gmail.com Ткачук А. А. Tkachuk A. A. Кладченко Е. С. Kladchenko E. S. Андреева А. Ю. Andreeva A. Yu. Институт биологии южных морей им. А.О. Ковалевского РАН Севастополь Россия A.O. Kovalevsky Institute of Biology of the South Seas of RAS Sevastopol Russian Federation Институт биологии южных морей им. А.О. Ковалевского РАН Севастополь Россия A.O. Kovalevsky Institute of Biology of the South Seas of RAS Sevastopol Russian Federation Институт биологии южных морей им. А.О. Ковалевского РАН Севастополь Россия A.O. Kovalevsky Institute of Biology of the South Seas of RAS Sevastopol Russian Federation Институт биологии южных морей им. А.О. Ковалевского РАН Севастополь Россия A.O. Kovalevsky Institute of Biology of the South Seas of RAS Sevastopol Russian Federation 06 06 2024 06 06 2024 8 4 505 509 29 07 2023 https://rusjbpc.ru/en/nauka/article/83718/view

В настоящей работе при помощи методов проточной цитометрии и лазерной дифракции проведен анализ функционального состояния гемоцитов (осмотическая хрупкость, соотношение типов клеток в гемолимфе, способность к продукции активных форм кислорода – АФК) средиземноморской мидии Mytilus galloprovincialis (Lamarck, 1819), акклиматизированных к низкой (6 ‰, 10 ‰, 14 ‰) и высокой солености (24 ‰, 30 ‰). Акклимация к различной солености приводила к разнонаправленному изменению клеточного состава гемолимфы мидий, наиболее выраженный эффект наблюдался при низкой солености. При этом, как в условиях низкой, так и высокой солености отмечен рост внутриклеточной концентрации АФК в гемоцитах, что может свидетельствовать о развитии окислительного стресса. Кроме этого, при всех изменениях солености был зафиксирован сдвиг кривой осмотической хрупкости. Результаты настоящей работы показали, что средиземноморская мидия обладает выраженной толерантностью к кратковременным (48 ч) колебаниям солености, при этом снижение солености сопровождалось более выраженными функциональными изменениями. В свою очередь сдвиг кривой осмотической стойкости свидетельствует об участии клеточных механизмов осморегуляции у средиземноморских мидий при адаптации к краткосрочному изменению солености.

In this work, using the methods of flow cytometry and laser diffraction, the analysis of the functional state of hemocytes (osmotic fragility, the ratio of cell types in the hemolymph, the ability to produce ROS) was carried out of the Mediterranean mussel Mytilus galloprovincialis (Lamarck, 1819), acclimatized to low (6 ‰, 10 ‰, 14 ‰) and high salinity (24%, 30 %). Acclimation to different salinity led to a multidirectional change in the cellular composition of the hemolymph of mussels, the most pronounced effect was observed at low salinity. At the same time, both in conditions of low and high salinity, an increase in the intracellular concentration of ROS in hemocytes was noted, which may indicate the development of oxidative stress. In addition, with all changes in salinity, a shift in the osmotic fragility curve was recorded. The results of this work showed that the Mediterranean mussel has a pronounced tolerance to short-term (48 h) fluctuations in salinity, while the decrease in salinity was accompanied by more pronounced functional changes. In turn, the shift in the osmotic resistance curve indicates the involvement of cellular mechanisms of osmoregulation in Mediterranean mussels in adapting to short-term changes in salinity.

 гемоциты клеточный состав гемолимфы активные формы кислорода соленость осмотическая хрупкость hemocytes hemolymph cellular composition reactive oxygen species salinity osmotic fragility Работа выполнена в рамках гранта РНФ № 22-26-00165 «Функциональный и иммунный статус двустворчатых моллюсков-объектов марикультуры в условиях действия факторов глобальных изменений климата».

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