Compensatory response of blastomeres in two-cell mouse embryo to hypoosmotic stress was studied employing the direct measurement of cellular volume with Laser Scanning Microtomography followed by 3-D reconstruction (QLSM). It was shown that embryos exposed to hypotonicity first swelled and then returned to the initial size. Swelling phase was defined by a water permeability coefficient (Lp) of 0.4 micron/(min.atm). The next compensatory phase of regulatory volume decrease (RVD) in embryonic cells was not dependent on Na+/K+-ATPase inhibition or low pH (6.4), but RVD was abolished by Cytochalasine B (Cyto B) treatment.
two-cell mouse embryo, regulatory volume decrease, Na+/K+-ATPase, Cytochalasine B, laser scanning microscopy, 3D-reconstruction
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