NOCTILUCA SCINTILLANS CELL SINGLE FLASH MODEL CONSIDERING BIOCHEMICAL BACKGROUND OF ITS LIGHT EMISSION
Rubrics: GENERAL BIOPHYSICS
Authors:
1.
Sevastopol State University
2.
A.O. Kovalevsky Institute of Marine Biological Research of RAS
3.
Sevastopol State University; A.O. Kovalevsky Institute of Marine Biological Research of RAS
Type:
Article
Pages:
from 108
to 111
Status:
Published
Received:
20.06.2016
Accepted:
20.06.2016
Published:
25.06.2016
Subject area:
UDK 577 Материальные основы жизни. Биохимия. Молекулярная биология.Биофизика
Language:
Russian
Keywords:
Noctiluca scintillans, bioluminescence, flash-triggering action potential, phenomenological model, enzyme-substrate interaction, Noctiluca scintillans
Abstract (English):
The present work reports the model of a single bioluminescent flash of Noctiluca scintillans cell considering bioelectrical trigger and biochemical background of light emission. A system of differential equations is suggested in order to obtain physicochemical parameters of bioluminescence and characteristics of the flash registered. A simple approach is put in the basis of model which involves luminescent protein luciferin to interact with luciferase enzyme. In addition, activation of the key compounds is taking into account provided by flash-triggering action potential propagation through thin peripheral layer of a cell cytoplasm. Minimization of discrepancy function was accomplished within two stages. At the first stage, an approximation of exponential part of bioluminescent flash decay was implemented in order to introduce empirical restraint on model parameters. At the second stage, an approximation of the full equations system was implemented bearing in mind empirical restraint applied to parameters on the previous stage. The model was tested using bioluminescence records of N. scintillans cells collected in Sevastopol region on April 14, 2011.
The present work reports the model of a single bioluminescent flash of Noctiluca scintillans cell considering bioelectrical trigger and biochemical background of light emission. A system of differential equations is suggested in order to obtain physicochemical parameters of bioluminescence and characteristics of the flash registered. A simple approach is put in the basis of model which involves luminescent protein luciferin to interact with luciferase enzyme. In addition, activation of the key compounds is taking into account provided by flash-triggering action potential propagation through thin peripheral layer of a cell cytoplasm. Minimization of discrepancy function was accomplished within two stages. At the first stage, an approximation of exponential part of bioluminescent flash decay was implemented in order to introduce empirical restraint on model parameters. At the second stage, an approximation of the full equations system was implemented bearing in mind empirical restraint applied to parameters on the previous stage. The model was tested using bioluminescence records of N. scintillans cells collected in Sevastopol region on April 14, 2011.
Keywords:
Noctiluca scintillans, bioluminescence, flash-triggering action potential, phenomenological model, enzyme-substrate interaction, Noctiluca scintillans
Noctiluca scintillans, bioluminescence, flash-triggering action potential, phenomenological model, enzyme-substrate interaction, Noctiluca scintillans
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