Article is devoted to application of DSC and small-angle X-ray scattering (SAXS) for study of structure of two types of experimental objects - multilammelar liposomes, formed from synthetic individual phospholipid, and another, formed from mixture of nature phospholipids. It is an actual task, because such experimental objects adequately reflect properties of multilayer biomembranes. Cellular fates depend on membrane structure - the first target for biologically active substances (BAS) which change membrane properties. The main thesis is that the multilammelar liposomes, formed from individual phospholipid or from natural mixture of phospholipids should be investigated by different methods characterizing different levels of structe organization. Multilamellar liposomes formed from individual phospholipid, as control sample and under the action of BAS, are appropriate objects for DSC and SAXS study. DSC registers thermodynamic parameters of main phospholipid phase transitions that characterize bilayer microdomains organization. Examining by DSC of natural phospholipids mixture does not give any complete information on thermodynamic parameters of individual main phase transitions of each component of the mixture. Researchers obtain the thermograms, without correct characteristics of transitions cooperativity, heat capacity, and temperature at transition peak for each individual lipid component. In SAXS study of liposomes formed from mixture of nature phospholipids researchers obtained adequate characteristic of structural properties of liposomes in control sample and under the action of BAS. By SAXS application the structure parameters of multilammelar liposomes are obtained at the higher organization levels concerning the thickness of bilayers and their packing in multilammelar liposome in control samples and under the action of BAS.
DSC, small-angle X-ray scattering (SAXS), phospholipids, multilammelar liposomes
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