The analysis of NMR spectroscopy data: dependence of chemical shift on the concentration of dissolved substances and [1H; 1H] NOESY spectra, of various combinations of MGB ligands Thiazotropsin A and B and the duplex oligonucleotide, having binding sites for the ligands of each type, allowed to determine the constants of self- and hetero- aggregation, and also it was suggested the nature of preassemble way of ligand homodimers binding DNA.
MGB ligand, DNA, complexation, three-component system
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