CONCEPT AND METHODOLOGICAL APPROACHES TO THE STUDY OF THE PROPERTIES OF NANOSTRUCTURED FRUSTULE OF DIATOMS (BACILLARIOPHYTA) TO IDENTIFY THE SPECIES
Abstract and keywords
Abstract (English):
The fine structure of marine planktonic diatom frustule Skeletonema sp. (Bacillariophyta), laboratory clone Sk021002, was studied using methods of electron microscopy and Raman spectroscopy. At the present work we were used the current methods of identification of the species. Using scanning and transmission electron microscopy showed that the structure of the shell - valve, marginal process and girdle bands were morphologically differ. The first time had been obtained by the method of Raman spectroscopy data of diatoms of the genius Sceletonema . The differences were found in the SERS spectra for organic casing and silicon frustule of clone Sk021002. Application of method by Raman spectroscopy provides extensive and unique information on the nanocharacteristics of the various elements of diatoms frustule. The study of biological objects by method of Raman spectroscopy is non-invasive, this is important moment for fundamental biophysical research.

Keywords:
identification, frustule, Raman spectroscopy, nanocharacteristics
Text
Publication text (PDF): Read Download
References

1. Barletta R.E., Krause J.W., Goodie T., Sabae H.E. The direct measurement of intracellular pigments in phytoplankton using resonance Raman spectroscopy. Marine Chemistry, 2015, vol. 176, pp. 164-173.

2. Griffith W.P. Advances in the Raman and infrared spectroscopy of minerals. Spectroscopy of inorganic - based materials. Eds R.J.H. Clark and R.E.Hester. John Wiley & Sons Ltd Press, 1987, pp. 119-186.

3. Hasle G.R., Fryxell G.A. Diatoms: cleaning and mounting for light and electron microscopy. Transactions of the American Microscopical Society, 1970, vol. 89, pp. 469-474.

4. John R. Ferraro, Kazuo Nakamoto and Chris W. Brown. Introductory Raman Spectroscopy. Academic Press, Amsterdam, Second Edition, 2003, 434 p.

5. Korolev E.V., Smirnov V.A., Zemlyakov A.N. Identifikaciya novoobrazovaniy, obuslovlennyh scheloche- silikatnoy reakciey. Vestnik MGSU. Nauchno-tehnicheskiy zhurnal, 2013, t. 6, s. 109-116. [Korolev E.V., Smirnov V.A., Zemlyakov A.N. Identification of Alkali-silica Reaction Outcomes. Vestnik MGSU. Proceedings of Moscow State University of Civil Engineering, 2013, vol. 6, pp. 109-116. (In Rus.)]

6. Lee R.E. Phycology, 4th edition. Cambridge: Cambridge University Press, 2008, 547 p.

7. Lošić D., Short K., Mitchell J.G., Lal R., Voelcker N.H. AFM nanoindentations of diatom biosilica surfaces. Langmuir, 2007, vol. 23, pp. 5014-5021.

8. Pletikapić G., Berquand A., Radić T.M., Svetlićic V. Quantitative nanomechanical mapping of marine Diatom in seawater using peak force tapping Atomic Force Microscopy. J. Phycol., 2012, vol. 48, pp. 174-185.

9. Round F.E., Crawford R.M., Mann D.G. The Diatoms. Biology and morphology of the genera. Cambridge, 1990, 747 p.

10. Sarno D., Kooistra W.H.C.F., Medlin L.K., Percopo I., Zingone A. Diversity in the genus Skeletonema (Bacillariophyceae): II. An assessment of the taxonomy of S. costatum-like species with the description of four new species. J. Phycol., 2005, vol. 41, pp. 151-176.

11. Zaharov V.P., Larin K, Bratchenko I.A. Povyshenie informativnosti opticheskoy kogerentnoy tomografii pri diagnostirovanii kozhnyh patologiy. Vestnik Samarskogo gosudarstvennogo aerokosmicheskogo universiteta, 2011, t. 2, c. 232-239. [Zaharov V.P., Larin K., Bratchenko I.A. Increasing the information content of optical coherence tomography skin pathology detection. Vestnik STAU, 2011, vol. 2, pp. 232-239. (In Rus.)]


Login or Create
* Forgot password?