Sorbents on the basis of natural zeolite modified with chitosan and chitosan with following addition of copper salts chitosan were prepared. The sizes and surface potentials of chitosan and endotoxins (LPS) of gram-negative bacteria were determinated in solution. Calculated binding constant of lipopolysaccharide to chitosan indicated a high affinity of their interaction. It was found the zeolite-chitosan composite and especially zeolite-chitosan-copper сomposite in solution bind from 60 to 99 % of endotoxin. These results were confirmed by LAL assay. It is shown that column with the proposed sorbents can be used for preparative producing of pyrogen-free solutions.
chitosan, zeolites, lipopolysaccharide, sorbtion of endotoxin
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