The features of variation in super weak luminescence intensity of tissues have been studied under different regimes of freezing and thawing. For the first time, super weak luminescence (hemiluminescence-HL) outbreaks of tissues were established at the temperature conditions under which fresh-steamed tissues don’t radiate. After freezing- thawing at -79 °C, HL outbreaks of liver, muscular and skin tissues were occurred at +5 °C, +8 °C and +10 °C, respectively. After freezing-thawing at -196 °C, HL outbreaks in the samples were occurred at relatively high temperatures and their amplitude were significantly lower than the amplitudes in the samples frozen at -79 °C. HL outbreaks after freezing-thawing at -196 °C in liver, muscular and skin tissues were occurred at +8 °C, +12 °C and + 14 °C, respectively. Shifting in temperature point of HL outbreaks at different freezing-thawing regimes is associated with both mechanical and osmotic destructions of tissue structure. It was established that oxidative stress plays significant role in the amplitude of outbreak. Taking this into account, before the freezing-thawing process tissue samples were treated with a solution containing 10 % glycerin and 0,2 % potassium-phenozane solutions. After such treatment, the temperatures of HL outbreaks were increased as following: in liver tissue by +11 °C, in muscular tissue by +12 °C and in skin tissue by +15 °C, and the protective solution significantly decreased the outbreaks amplitude. The data derived from experiments formed an idea about the mechanism of tissue preservation intended for transplantation after freezing-thawing.
super weak luminescence, spontaneous hemiluminescence, freezing-thawing, protective substances
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