The dynamics of the growth of microalgae Tetraselmis viridis in the butch culture has been experimentally determined. The maximum productivity and maximum density of the culture under photoautotrophic and mixotrophic cultivation have been determined. It is shown that the microalgae growth in the media with organic carbon source is higher compared to growth without organic carbon. The maximum productivity (0.28 g АСВ/(l·day)) and maximum biomass (3.1 g АСВ/l) of culture were higher in 1.5 times under microalgae’s culture with organic carbon and energy sources compared to photoautotrophic growth. It has been concluded that the addition of organic carbon sources in media resulting in improved growth of photosynthetic organisms.
Tetraselmis viridis, butch culture, carbon, maximum productivity, density of the culture
1. Bigon M., Harper Dzh., Taunsend K. Ekologiya. Osobi, populyacii i soobschestva: V 2-h t. T.2: Per. s angl. M.: Mir, 1989, 160 s. [Begon M., Harper J., Townsend C. Ecology. Individuals, Populations and Communities: In 2 Vols. Volume 2: Tr. from English. M.: Mir, 1989, 160 p. (In Russ.)]
2. Borowitzka M.A., Borowitzka L.J. Vitamins and fine chemicals from micro-algae. Micro-algal biotechnology. 1988, pp. 153-196.
3. Wen Z.-Y., Chen F. Production potential of eicosapentaenoic acid by the diatom Nitzschia laevis. Biotechnology Letters, 2000, vol. 22, no. 9, pp. 727-733.
4. Heredia-Arroyo T., Wei W., Ruan R., Hu B. Mixotrophic cultivation of Chlorella vulgaris and its potential application for the oil accumulation from non-sugar materials. Biomass and Bioenergy, 2011, vol. 35, no. 5, pp. 2245-2253.
5. Azma M., Mohamed M.S., Mohamad R., Rahim R.A., Arif A. B. Improvement of medium composition for heterotrophic cultivation of green microalgae Tetraselmis suecica, using response surface methodology. Biochemical Engineering Journal, 2011, vol. 53, no. 2, pp. 187-195.
6. Fang X., Wei C., Zhao-Ling C., Fan O. Effects of organic carbon sources on cell growth and eicosapentaenoic acid content of Nannochloropsis sp. Journal of Applied Phycology, 2004, vol. 16, no. 6, pp. 499-503.
7. Garcia M.C., Camacho F.G., Miron A.S., Sevilla J.M.F., Chisti Y., Grima E.M. Mixotrophic production of marine microalga Phaeodactylum tricornutum on various carbon sources. J. Microbiol. Biotechnol, 2006, vol. 16, no. 5, pp. 689-694.
8. Wood B.J.B., Grimson P.H.K., German J.B., Turner M. Photoheterotrophy in the production of phytoplankton organisms. Journal of Biotechnology, 1999, vol. 70, no. 1-3, pp. 175-183.
9. Xu F., Hu H.-H., Cong W., Cai Z.-L., Ouyang F. Growth characteristics and eicosapentaenoic acid production by Nannochloropsis sp. in mixotrophic conditions. Biotechnology Letters, 2004, vol. 26, no. 1, pp. 51-53.
10. Orosa M., Franqueira D., Cid A., Abalde J. Carotenoid accumulation in Haematococcus pluvialis in mixotrophic growth. Biotechnology Letters, 2001, vol. 23, no. 5, pp. 373-378.
11. Trenkenshu R.P. Prosteyshie modeli rosta mikrovodorosley. 1. Periodicheskaya kul'tura. Ekologiya morya, 2005, t. 67, s. 89-97. [Trenkenshu R.P The simplest models of microalgae growth. 1. Periodic culture. Sea Ecology, 2005, vol. 67, pp. 89-97 (In Russ.)]
12. Trenkenshu R.P. Rostovye i fotoenergeticheskie harakteristiki morskih mikrovodorosley v plotnoy kul'ture: avtoref. diss. … kand. biol. nauk: spec. 03.00.02 «Biofizika». Krasnoyarsk, 1984, 28 s. [Trenkenshu R.P Growth and photoenergy characteristics of marine microalgae in a dense culture: Abstract. diss. … cand. biol. Sciences: Spec. 03.00.02 "Biophysics". Krasnoyarsk, 1984, 28p. (In Russ.)]
13. Trenkenshu R.P., Borovkov A.B., Lelekov A.S. Unificirovannaya laboratornaya ustanovka dlya issledovaniya nizshih fototrofov. Sevastopol': Izd-vo InBYuM NANU, 2009, 40 s. [Trenkenshu R.P, Borovkov A.B, Lelekov A.S. Standardized laboratory installation for the study of the lower phototrophs. Sevastopol: Publishing House of the IBSS, 2009, 40 p. (In Russ.)]
14. Mirón A.S., Garcia M.C., Gómez A.C., Camacho F.G., Grima E.M., Chisti, Y. Shear Stress Tolerance and Biochemical Characterization of Phaeodactylum tricornutum in Quasi Steady-state Continuous Culture in Outdoor Photobioreactors. Biochem. Eng. J., 2003, vol. 16, pp. 287-297.
15. Borovkov A.B., Gevorgiz R.G. Produktivnost' Spirulina platensis i Tetraselmis viridis pri ispol'zovanii razlichnyh metodov kul'tivirovaniya. Ekologiya morya, 2005, t. 70, s. 9-13. [Borovkov A.B., Gevorgiz R.G. Productivity of Spirulina platensis and Tetraselmis viridis by using different methods of cultivation. Sea Ecology, 2005, vol. 70, pp. 9-13. (In Russ.)]
16. Lelekov A.S., Trenkenshu R.P. Prosteyshie modeli rosta. 4. Eksponencial'naya i lineynaya fazy rosta. Ekologiya morya, 2007, vyp. 74, s. 47-49. [Lelekov A.S, Trenkenshu R.P The simplest models of growth. 4. Exponential and linear growth phases. Sea Ecology, 2007, vol. 74, pp. 47-49. (In Russ.)]
17. Wen Z.Y., Chen F. Production potential of eicosapentaenoic acid by the diatom Nitzschia laevis. Biotechnology Letters, 2000, vol. 26 (1), pp. 727-733.
