The decay kinetics of erythrosine delayed luminescence in malignant and healthy biological tissues of laboratory mice was investigated. Upon periodical pulse excitation of luminescence an effect of delayed fluorescence suppression was discovered. The glow quenching value is dependent on the laser pulses repetition period. It is shown that the effect is associated with the decrease in the contribution of luminescence via photogenic annihilation reaction of triplet sensitizer and singlet oxygen excited states. In addition, the oxygen-dependent fluorescence decay affecting to the phosphorescence intensity is discussed. The obtained data suggest that it is possible to evaluate intensive oxygen consumption into a tissue using annihilation delayed fluorescence.
delayed fluorescence, biological tissues, singlet-triplet annihilation, singlet oxygen
1. Krasnovsky A. A. (Jr.) Primary Mechanisms of Photoactivation of Molecular Oxygen. History of Development and the Modern Status of Research. Biochemistry (Moscow), 2007, vol. 72, pp. 1065-1080.
2. Letuta S. N., Kuvandykova A. F., Pashkevich S. N., Saletskii A. M. Features of the delayed fluorescence kinetics of exogenous fluorophores in biological tissues. Russian Journal of Physical Chemistry A, 2013, vol. 87, pp. 1582-1587.
3. Kucherenko M.G. Kinetika molekulyarnyh fotoprocessov. Postanovka i reshenie zadach. Orenburg: OOO IPK «Universitet», 2012, 190 c. [Kucherenko M.G. Kinetics of molecular photo processes. Problem formulation and solutions. Orenburg: OOO IPC «Universitet», 2012, 190 p. (In Russ.)].
4. Berezin M.Y., Achilefu S. Fluorescence lifetime measurements and biological imaging. Chem. Rev., 2010, vol. 110, pp. 2641-2684.
5. Plaetzer K., Krammer B., Berlanda J., Berr F., Kiesslich T. Photophysics and photochemistry of photodynamic therapy: fundamental aspects. Lasers Med. Sci., 2009, vol. 24, pp. 259-268
6. Uzdenskiy A.B. Kletochno-molekulyarnye mehanizmy fotodinamicheskoy terapii. SPb.: Nauka, 2010, 192 c. [Uzdensky A.B. Cell and molecular mechanisms of photodynamic therapy. Saint-Peterburg: Nauka, 2010, 129 p. (In Russ.)]
7. Snyder J.W., Skovsen E., Lambert J.D.C., Poulsen L., Ogilby P.R. Optical detection of singlet oxygen from single cells. Phys. Chem. Chem. Phys., 2006, vol. 8, pp. 4280-4293
8. Li B., Lin H., Chen D., Wilson B.C., Gu Y. Singlet oxygen detection during photosensitization. J. Innov. Opt. Health Sci., 2013, vol. 6, pp. 1330-1332.
9. Papkovsky, D., Zhdanov, A.V., Fercher, A., Dmitriev, R., Hynes, J. Phosphorescent Oxygen-Sensitive Probes. Springer Briefs in Biochemistry and Molecular Biology, 2012, pp. 1245-1255.
10. Dmitriev R.I., Papkovsky D.B. Optical probes and techniques for O2 measurement in live cells and tissue. Cell. Mol. Life Sci., 2012, vol. 69, pp. 2025-2039.
11. Scholz M., Dědic R., Valenta J., Breitenbach T., Hàla J. Real-time luminescence microspectroscopy monitoring of singlet oxygen in individual cells. Photochem. Photobiol. Sci., 2014, vol. 13, pp. 1203-1212.
12. Letuta S.N., Kuvandykova A.F., Pashkevich S.N. The Kinetics of Exogenous Phosphors Delayed Fluorescence in Tissues. J. Anal. Oncology, 2012, vol. 1, pp. 107-110.
13. Moiseeva E.V. Original approaches to test antibreast cancer drugs in a novel set of mouse models. Utrecht: Proefschrift Universiteit Utrecht, 2005, 191 p.
14. Veuthey T., Herrera G., Dodero V.I. Dyes and Stains: from molecular structure to histological application. Frontiers in Bioscience, 2014, vol. 19, pp. 91-112.
15. Baker A., Kanofsky J.R. Quenching of singlet oxygen by biomolecules from L1210 leukemia cells. Photochem. Photobiol., 1992, vol. 55, p. 523.
