The analytical systems for specific microorganism detection were developed using streptavidin- luciferase Luciola mingrelica fusion protein (His6-SA-Luc). Heterogeneous immunoassay is proposed for detection of Salmonella cells using monoclonal biotinylated antibodies in range 104-5×106 CFU/ml. We optimized hybridization analysis of a specific DNA fragment from E. coli. The detection limit for DNA was 10-10 M. We showed that His6-SA-Luc fusion protein is a highly sensitive and specific reagent for microorganism detection based on biotin- streptavidin interaction using or ELISA, or hybridization analysis of a specific DNA fragment from microbial cells.
Salmonella, bioluminescence, ELISA, streptavidin-luciferase, fusion, Luciola mingrelica, hybridization analysis, Salmonella
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