An analytical equation for exponential bioluminescence decay rate calculation was obtained using photobiochemical background of the light emission of Noctiluca scintillans. The equation was derived for light emission times elapsing well after flash initiation in order to avoid an influence of nonlinear process such as flash-triggering action potential propagation through thin peripheral layer of cell cytoplasm of Noctiluca scintillans . The equation derived imposes an additional constraint on Noctiluca scintillans flash model parameters enabling one to exclude one of the six optimization parameters while flash curve approximation by theoretical model. Analysis allows us to conclude dependence of exponential decay rate of photoemission on cell physiological state, that is for the same Noctiluca scintillans cell one can register flashes with different bioluminescence decay. Such a conclusion is confirmed by prior studies.
Noctiluca scintillans, bioluminescence, flash-triggering action potential, enzyme-substrate interaction, Noctiluca scintillans
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