The changes of the enzymes activity (G6FDH, GR, LDH, MDH) in Ehrlich ascites carcinoma cells at different stages of tumor growth after exposure to microwave radiation have been studied. It is shown that the change in the activity of enzymes depends not only on the effect of microwave radiation, but on the characteristics of the metabolism of tumor cells, which are associated with the tumor growth stage in the mice organism. The observed changes in the activity of enzymes indicate discrepancy in the regulation of anaerobic and aerobic metabolism in Ehrlich ascites carcinoma cells in the microwave radiation exposure. Under microwave radiation the changes in the activity of G6PDH and GR are inconsistent, which leads to a decrease in the cell ability to resist the processes of generation of reactive oxygen.
dehydrogenase, enzyme activity, electromagnetic radiation, microwave radiation, cell phone, Ehrlich ascites carcinoma
1. Kochemarova E.V., Kochemarova Yu.V., Kruglik O.V., Morgulis I.I. Elektromagnitnaya nagruzka na cheloveka i prirodu. Inzhenernaya ekologiya, 2012, № 6, s. 35-46. [Kochemarova E.V., Kochemarova Ju.V., Kruglik O.V., Morgulis I.I. The effect of electromagnetic fields on biological objects. Inzhenernaya ekologiya, 2012, no. 6, pp. 35-46. (In Russ.)]
2. Grigor'ev Yu.G. Principial'no novoe elektromagnitnoe zagryaznenie okruzhayuschey sredy i otsutstvie adekvatnoy normativnoy bazy - k ocenke riska (analiz sovremennyh otechestvennyh i zarubezhnyh dannyh). Gigiena i sanitariya, 2014, № 3, s. 11-16. [Grigor’ev Ju.G. Fundamentally new electromagnetic pollution and the lack of adequate regulatory framework - on the risk assessment (analysis of modern domestic and foreign data). Gigiena i sanitariya, 2014, no. 3, pp. 11-16. (In Russ.)]
3. Grigor'ev Yu.G., Biryukov A.P. Radiobiologiya mobil'noy svyazi: sovremennye aspekty fundamental'nyh i prikladnyh issledovaniy. Medikobiologicheskie problemy zhiznedeyatel'nosti, 2014, № 1 (11), s. 6-16. [Grigor’ev Ju.G., Birjukov A.P. [Radiobiology of mobile communication: Modern aspects of fundamental and applied research. Mediko-biologicheskie problemy zhiznedeyatel’nosti, 2014, no. 1 (11), pp. 6-16. (In Russ.)]
4. Markov M., Grigoriev Y.G. Wi-Fi technology - an uncontrolled global experiment on the health of mankind. Electromagnetic Biology and Medicine, 2013, vol. 32, no. 2, pp. 200-208.
5. Gaestel M. Biological monitoring of non-thermal effects of mobile phone radiation: recent approaches and challenge. Biological Reviews, 2010, vol. 85, no. 3, pp. 489-500.
6. Ozaslan M., Karagoz I.D., Kilic I.H., Guldur M.E. Ehrlich ascites carcinoma. African Journal of Biotechnology, 2013, vol. 10, no. 13, pp. 2375-2378.
7. Savchenko A.A. Biolyuminescentnoe opredelenie aktivnosti NAD- i NADF-zavisimyh glutamatdegidrogenaz limfocitov. Laboratornoe delo, 1991, № 11, s. 22-25. [Savchenko A.A. Bioluminescent determination of the activity of NAD- and NADP-dependent glutamate dehydrogenases of lymphocytes Laboratornoe delo, 1991, no. 11, pp. 22-25. (In Russ.)]
8. Gatenby R.A., Gillies R.J. Why do cancers have high aerobic glycolysis? Nat Rev Cancer. 2004, vol. 4, pp. 891-899.
9. Talaiezadeh A., Shahriari A., Tabandeh M.R., Fathizadeh P., Mansouri S. Kinetic characterization of lactate dehydrogenase in normal and malignant human breast tissues. Cancer Cell Int., 2015, vol. 15, p.19.
10. DeBerardinis R.J., Lum J.J., Hatzivassiliou G., Thompson C.B. The biology of cancer: Metabolic reprogramming fuels cell growth and proliferation. Cell Metab., 2008, vol. 7, pp. 11-20.
11. Stanton R.C. Glucose-6-Phosphate Dehydrogenase, NADPH, and cell survival. International Union of Biochemistry and Molecular Biology Life, 2012, vol. 64, no. 5, pp. 362-369.
12. Diaz-Flores M., Ibanez-Hernandez M.A., Galvan R.E., Gutierrez M., Duran-Reyes G., Medina-Navarro R., Pascoe-Lira D., Ortega-Camarillo C., Vilar-Rojas C., Cruz M., Baiza-Gutman L.A Glucose-6-phosphate dehydrogenase activity and NADPH/NADP+ ratio in liver and pancreas are dependent on the severity of hyperglycemia in rat. Life Sciences, 2006, vol. 78, pp. 2601-2607.
13. Schafer F.Q., Buettner G.R. Redox environment of the cell as viewed through the redox state of the glutathione disulfide/glutathione couple. Free Radical Biology & Medicine, 2001, vol. 30, no. 11, pp. 1191-1212.
14. Lupez-Mirabal H.R., Winther J.R. Redox characteristics of the eukaryotic cytosol. Biochimica et Biophysica Acta, 2008, vol. 1783, pp. 629-640.
15. Barron J.T., Gu L., Parrillo J.E. NADH/NAD redox state of cytoplasmic glycolytic compartments in vascular smooth muscle. American Journal of Physiology - Heart and Circulatory Physiology, 2000, vol. 279, pp. H2872-H2878.
16. Inzhevatkin E.V., Savchenko A.A. The nonspecific metabolic reaction of cells to extreme exposures. Biology Bulletin., 2016, vol. 43, no. 1, pp. 2-11.
17. Inzhevatkin E.V., Fomenko E.Yu., Slepov E.V., Savchenko A.A. Metabolic changes of lymphocytes and neoplastic cells in mice with Ehrlich ascites carcinoma during tumor growth. Biology Bulletin, 2007, vol. 34, no. 3, pp. 310-313.
18. Fischer K., Hoffmann P., Voelkl S., Meidenbauer N., Ammer J., Edinger M., Gottfried E., Schwarz S., Rothe G., Hoves S. Inhibitory effect of tumor cell-derived lactic acid on human T cells. Blood, 2007, vol. 109, pp. 3812-3819.
19. Martinez-Outschoorn U.E., Lin Z., Trimmer C., Flomenberg N. Cancer cells metabolically ‘fertilize’ the tumor microenvironment with hydrogen peroxide, driving the Warburg effect: Implications for PET imaging of human tumors. Cell Cycle, 2011, vol. 10, pp. 2504-2520.
20. Swietach P., Vaughan-Jones R.D., Harris A.L. Regulation of tumor pH and the role of carbonic anhydrase. Cancer Metastasis Rev, 2007, vol. 26, pp. 299-310.
21. Zakhvataev V.E., Khlebopros R.G. The Kupershtokh-Medvedev electrostrictive instability as possible mechanism of initiation of phase transitions, domains and pores in lipid membranes and influence of microwave irradiation on cell. Biophysics, 2012, vol. 57, no. 1, pp. 61-67.
