Delayed luminescence kinetics of xanthene dyes was investigated for biological tissues with various oxygen tensions. It was shown that a large part of sensitizer delayed fluorescence is generated through annihilation of triplet-excited sensitizer and singlet oxygen (STA-DF) when samples are in open air. If tissue oxygen tension decreases including as a result of the photodynamic effect, quenching of STA-DF is observed. It is shown that the STA-DF has higher sensitivity to singlet oxygen amount in the tissue than other types of sensitizer delayed luminescence. It is proposed to monitor the oxygen dynamics in the tissue by the magnitude of the STA-DF intensity in real time. Some advantages of this approach over alternative fluorescent techniques of tissue oxygen monitoring are discussed.
singlet oxygen, biological tissue oxygenation, singlet-triplet annihilation, delayed fluorescence
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