Urolithiasis refers to the most common, severe and often recurring urological diseases, representing a serious problem with social significance [1]. The formation of urinary stones is associated with physicochemical processes in which the threshold solubility of salts decreases or their concentration exceeds the threshold solubility [2]. However, there are factors that prevent the crystallization of urinary salts in the urine. The main one is the Tamm-Horswall protein (THP). Previously, it was being shown that THP under different conditions can exist in different oligomeric forms [9,10]. The purpose of this work was to assess the THP desialisation degree in terms of the Z-potential and to search for differences between patients with urolithiasis and those without a urinary system pathology. Measurements of the THP Z-potential showed that in healthy donors the distribution of THP particles along the Z-potential is unimodal. The average value of the THP Z-potential in healthy donors was 28.4 mV. In patients with urolithiasis, the Z-potential distribution was bimodal. The average values of the modes were - 29.7 mV b - 6.2 mV. The paper discusses the relationship between the degree of desialization with oligomeric forms of BTX and the possible inhibition or sensitization process mechanisms stone formation.
Urolithiasis, Tamm-Horsfall protein, Oligomerous forms, Zeta-potential, Sialic acids, Kidney stones
1. Dzeranov N.K., Zaharov V.N., Baybarin K.A. Sovremennye litotriptory dlya distancionnoy litotripsii mochevyh kamney. Lechaschiy vrach, 2005, № 7, s. 72-76. [Dzeranov N.K., Zakharov V.N., Baibardin K.A. Modern lipotriptors for distant lipotritions of urinary stones. Lechaschiy vratch, 2005, no. 7, pp 72-76. (In Russ.)]
2. Edinyy Yu.T., Dzyurak B.C., Zheltovskaya N.I. Koncentraciya vodorodnyh ionov mochi kak odin iz patogeneticheskih faktorov mochekamennoy bolezni. Vrachebnoe delo, 1987, № 12, c. 57-59. [Ediny U.T., Dzurak V.S., Geltovskaja N.I. Urinary pH is a one of pathogenic factors of urolitiasys. Vrachebnoe delo, 1987, no. 12, pp. 57-59. (In Russ.)]
3. Wesson J.A., Ward M.D. Pathological biomineralization of kidney stones. Elements, 2007, vol. 3, pp. 415-421.
4. Grover P.K., Moritz R.L., Simpson R.J., Ryall R.L. Inhibition of growth and aggregation of calcium oxalate crystals in vitro: A comparison of four human proteins. Eur. J. Biochem., 1998, vol. 253, pp. 637-644.
5. Beshensky A.M., Wesson J.A., Worcester E.M., Sorokina E.J., Snyder C.J., Kleimann J.G. Effects of urinary macromolecules on hydroxyapatite crystal formation. J. Am. Soc. Nephrol., 2001, vol. 12, pp. 2108-2116.
6. Hallson P.C., Choong S.K., Kasidas G.P., Samuell C.T. Effects of Tamm-Horsfall protein with normal and reduced sialic acid content upon the crystallization of calcium phosphate and calcium oxalate in human urine. Br. J. Urol., 1997, vol. 80, pp. 533-538.
7. Chen W.C., Lin H.S., Chen H.Y., Shin C.H., Li C.W. Effects of Tamm-Horsfall protein and albumin on calcium oxalate crystallization and importance of silaic acids. Mol. Urol., 2001, vol. 5, pp. 1-5.
8. Williams J., Marshall R.D., van H.H., Vliegenthart J.F. Structural analysis of the carbohydrate moieties of human Tamm-Horsfall glycoprotein. Carbohydr Res., 1984, vol. 134, pp. 141-155.
9. Landa S.B., Al'-Shukri S.H., Gorbachev M.I., Egorov V.V., Emanuel' V.L., Emanuel' Yu.V. Patohimicheskie osobennosti oligomernyh form belka Tamma-Horsvalla pri urolitiaze. Klinicheskaya laboratornaya diagnostika, 2016, t. 61, № 6, s. 335-342. [Landa S.B., Al-Shukri S.H., Gorbachev M.I., Egorov V.V., Emanuel Yu.V., Emanuel V.L. The pathochemical characteristics of oligomeric forms of Tamm-Horsfall protein under urolithiasis. Clin. Lab. Diag., 2016, vol. 61, no. 6, pp. 335-342. (In Russ.)]
10. Emanuel' V.L., Landa S.B., Emanuel' Yu.V., Izmaylov M.R. Patofiziologicheskaya interpretaciya biofizicheskoy modulyacii patohimicheskih form osnovnogo proteoma mochi pri urolitiaze. Laboratornaya sluzhba, 2017, t. 6, № 2, s. 21-27. [Emanuel V.L., Landa S.B., Emanuel Yu.V., Izmaylov M.R. Pathophysiological interpretation of biophysical modulation of basic urine proteome patochemical forms in case of urolithiasis. Laboratornaya sluzhba, 2017, vol. 6, no. 2, pp. 21-27. (In Russ.)]
11. Konya E., Amasaki N., Umekawa T., Iguchi M., Kurita T. Influence of urinary sialic acid on calcium oxalate crystal formation. Urol Int., 2002, vol. 68, pp. 281-285.
12. Youhanna S., Weber J., Beaujean V., Glaudemans B., Sobek J., Devuyst O. Determination of uromodulin in human urine: influence of storage and processing. Nephrol Dial Transplant., 2014, vol. 29, no. 1, pp. 136-145.
13. Trashkov A.P., Vasil'ev A.G., Kovalenko A.L., Tagirov N.S. Metabolicheskaya terapiya mochekamennoy bolezni na razlichnyh modelyah porazheniya pochek u krys. Eksperimental'naya i klinicheskaya farmakologiya, 2015, t. 78, № 3, s. 17-21. [Trashkov A.P., Vasiliev A.G., Kovalenko A.L., Tagirov N.S. Metabolic therapy of nephrolithiasis in two different rat models of kidney desease. Experimental and clinical pharmacology, 2015, vol. 78, no. 3, pp. 17-21. (In Russ.)]
14. Nicoli D., Chang Yu-Jain, Wu Jau-Sien Methods and apparatus for electrophoretic mobility determination using phase light scattering analysis. Patent US 7295311 B2, Date of publication 13.11.2007.
15. Serafini-Cessi F., Bellabarba G., Malagolini N., Dall’Olio F. Rapid isolation of Tamm-Horsfall glycoprotein (uromodulin) from human urine. J Immunol Methods, 1989, vol. 120, pp. 185-189.
16. Viswanathan P., Rimer J.D., Kolbach A.M., Ward M.D., Kleinman J.G., Wesson J.A. Calcium oxalate monohydrate aggregation induced by aggregation of desialylated Tamm-Horsfall protein. Urol Res., 2011, vol. 39, no. 4, pp. 269-282.
17. Hess B., Nakagawa Y., Parks J.H., Coe F.L. Molecular abnormality of Tamm-Horsfall glycoprotein in calcium oxalate nephrolithiasis. Am J Physiol., 1991, vol., 260, pp. F569-F578.
18. Mo L., Liaw L., Evan A.P., Sommer A.J., Lieske J.C., Wu X.R. Renal calcinosis and stone formation in mice lacking osteopontin, Tamm-Horsfall protein, or both. Am J Physiol Renal Physiol., 2007, vol. 293, pp. F1935-F1943.
19. Hallson P.C., Rose G.A. Uromucoids and urinary stone formation. Lancet, 1979, vol. 1, pp. 1000-1002.
20. Greenwood R, Kendall K. Electroacoustic studies of moderately concentrated colloidal suspensions. Journal of the European Ceramic Society, 1999, vol. 19, no. 4b, pp. 479-488.
