The discussion about biologically active substance (BAS) actions was presented. The using of different BAS concentrations may changes the vectors of BAS actions to biologic objects greatly. The mechanisms of BAS actions to structures and functions of biologic objects also were in largely depending on concentration of applied substances. In the case with hydrophilic melafen (strong plant growth regulator) we observed the changes of thermodynamic parameters of phospholipid multilammelar liposomes that testify of variable effects of different melafen concentrations on the organization of lipid microdomains in structure of homogeneous membrane. When studying the melafen actions on functions we found the concentration-depended vector’s change of melafen effects to the calcium signaling in cells of ascetic Ehrlich carcinoma. In the case with hydrophobe IHFAN-С10 (float hybrid antioxidant - the cholinesterase inhibitor, inserted in hydrophobe/hydrophilic phase of membranes) the ethanol-aqueous emulsions, when it was great diluted, change the mechanisms of IHFAN-С10 actions. Mechanisms varied from nonspecific destruction of membrane up to strengthening of membrane structure. We should indicate that in case of BASes - melafen and IHFAN-10, the certain different concentrations were achieved by the consistent dilutions of ethanol or aqua BAS solutions when strong mixing. But these concentrations were not measured by physical-chemical methods. It is reasonable to speak about dilutions, but not concentrations. Conclusion: it is necessary to take into account, when the BAS effects to any biologic objects over a wide range of BAS concentrations were investigated, the mechanism of action may be in great dependence of BAS degree diluted.
10, multilammelar liposomes, DSK, Erlich ascetic carcinoma, metabolic pathways, Ca2+-signaling
1. Fattahov S-G.G., Reznik V.S., Konovalov A.I. Melaminovaya sol' bis(gidroksimetil)fosfinovoy kisloty (Melafen), kak novyy sil'nyy regulyator rosta rasteniy. Materialy 13 internacional'noy konferencii himii fosfornyh soedineniy. S-Peterburg, 2002, c. 80. [Fattachov S.G., Reznik V.S., Konovalov A.I. In set of articles. Reports of 13th international conference on chemistry of phosphorus compounds, S. Petersburg, 2002, p. 80. (In Russ.)]
2. Nikiforov G.A., Belostockaya V.B., Vol'eva I.S., Komissarova N.L., Gorbunov D.B. Bioantioksidanty "poplavkovogo" tipa na osnove proizvodnyh 2,6 ditretbutil-fenola. V sb.: Bioantioksidant, Nauchnyy vestnik med akad., 2003, s. 50-51. [Nikiforov G.A., Vol,eva V.B., Belostotskaya I.S, Komissarova N.L., Gorbunov D.B. Bioantioxidants float type at the base of derivatives 2,6 ditertbutil-phenol. In set of articles “Bioantioxidant” Scientific Gerald of Medical Academic, 2003, pp. 50-51. (In Russ.)]
3. Zhigacheva I.V., Fatkullina L.D., Burlakova E.B., Shugaev A.I., Generozova I.P., Fattahov S-G.G., Konovalov A.I. Vliyanie fosfoorganicheskogo regulyatora rosta rasteniy na strukturnye harakteristiki membran rastitel'nogo i zhivotnogo proishozhdeniya. Biol. Membrany, 2008, t. 25, № 2, s. 150-156. [Zhigacheva I.V., Fatcullina L.D., Burlakova E.B., Shugaev A.I., Generosova I.P., Fattahov S.G., Konovalov A.I. Biological membrane, 2008, vol. 25, no. 2, pp. 150-156. (In Russ.)]
4. Alekseeva O.M., Krementsova A.V., Kim Yu.A., Krivandin A.V., Shatalova O.V., Zaikov G.E. Melafen effects to structural parameters of DMPC and egg phopholipids membranes. Vestnik Kazan Technology University, 2015, vol. 18, no. 2, pp. 14-22.
5. Alekseeva O.M., Krivandin A.V., Shatalova O.V., Krementsova A.V., Kim Yu.A. Thermodynamic and structural Parameters of Phospholipid Membranes. In book “Compositional Analysis of Polymers An Engineering Approach. Polimer surfaces and interfaces” Editor: M.S. Adaramola, O.V. Stoyanov, G. Zaikov, R.M. Akhmetkhanov, 2015, Chapter 2. Apple Academic Press.
6. Fatkullina L.D., Vekshina O.M., Burlakova E.B., Goloshchapov A.N., Kim Yu.A. Stabilization of cell membranes by hybrid antioxidants in therapy of neurodegenerative diseases. In book “In Biotechnology: State of the Art and Prospects for Development” Nova Science Publishers, New York, 2008. Ed. by P.E.Stott and G.E.Zaikov, Chapter 13, pp. 115-123.
