Pushchino, Moscow, Russian Federation
It was shown that a 1.5 hour-long exposure of mouse peritoneal neutrophils to a combination of a weak constant magnetic field (42 μT) and low-frequency alternating magnetic field collinear to the weak constant magnetic field (the sum of the frequencies 1.0, 4.4, and 16.5 Hz; amplitude, 0.86 μT) at physiological temperatures caused an increase in the intracellular production of reactive oxygen species, as measured by the changes in fluorescence of the products of 2,7-dichlorodihydrofluorescein and dihydrorhodamine 123 oxidation. The effect of weak magnetic fields was significantly more pronounced in the presence of low concentrations of respiratory burst activators (N-formyl-Met-Leu-Phe or phorbol-12-meristate-13-acetate). In contrast, exposure of neutrophils under magnetic shielding in hypomagnetic conditions (residual static magnetic field of 20 nT) causes a decrease in intracellular production of reactive oxygen species. The effect of the hypomagnetic field is observed after respiratory burst activators applied at low concentrations are additionally added.
magnetic field, hypomagnetic field, neutrophils, blood, reactive oxygen species, fluorescence
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