A design of a separation device and a method for microbiological samples separation based on barrier contactless dielectrophoresis are proposed. The principle of operation of the separation device is based on the formation of dielectrophoretic barriers in the volume of the separation chamber. The suggested method is highly efficient and easy to use in comparison with traditional planar dielectrophoresis systems. The feature of the method is the absence of contact between the separated samples and the conductive elements of the electrode structure. A computer simulation of the distribution of electric field over the dielectrophoretic barriers was carried out, and the effect of the thickness of the insulating coating on the dielectrophoretic properties of the barriers was shown. Based on the computer simulation data, the forces acting on the particle (a yeast cell with a diameter of 7 μm) in the separation chamber were estimated. The possibility of cell retention on cDEP barriers is theoretically predicted and experimentally confirmed using the example of yeast Saccharomyces cerevisiae .
cell separation, barrier contactless dielectrophoresis
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