The noncovalent interaction of a number of anionic meso-substituted carbocyanine dyes - 3,3'-di-(γ-sulfopropyl)-4,5,4',5'-dibenzo-9-methylthiacarbocyanine betaine (DMC), 3,3'-di-(γ-sulfopropyl)-4,5,4',5'-dibenzo-9-ethylthiacarbocyanine betaine (DEC), 3,3'-di-(γ-sulfopropyl)-5,5'-diphenyl-9-ethyloxacarbon-cyanine betaine (OEC) with human serum albumin (HSA) was studied by spectral-fluorescent methods. Substantial changes in the absorption and fluorescence spectra of the dyes in the presence of HSA indicate the formation of stable noncovalent complexes. Interaction with HSA leads to a significant increase in fluorescence of the dyes. It is shown that the interaction of meso-substituted dyes with HSA leads to a shift of cis-trans-isomeric equilibrium of the dyes. The dyes were proposed for use as spectral-fluorescent probes for evaluating the structural and functional properties of HSA and for analyzing the extracellular matrix in order to determine albumin whose dynamics of change can characterize the tissue under study during its development, the stage of growing up and aging.
thiacarbocyanine dyes, oxacarbocyanine dyes, serum albumin, noncovalent complex, spectral-fluorescent probes
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