FRC KSC SB RAS (International Scientific Center for Studies of Extreme States of Organism)
Krasnoyarsk, Krasnoyarsk, Russian Federation
In the model experiments in vitro , the applicability of EPR spectrometry for the detection of the modified detonation nanodiamonds (MNDs) in biomaterial samples (blood and homogenates of mouse organs) was established. The intensity of a characteristic EPR signal (g = 2.003, ΔH ≈ 10 G) registered in the samples increases linearly at MNDs concentrations in the range of 1.6-200 μg/ml. It was established in experiments in vivo that 2.5 hours after an intravenous injection to mice MNDs were mainly accumulated in the lungs and liver of animals - about 25 % and 20 % of the injected dose, respectively. A much smaller amount of MNDs (by 1-1.5 orders of magnitude) was found in the heart and kidneys. The presence of MNDs in the blood, spleen, brain and hip muscles was not detected within the sensitivity of the EPR method. Ten days after injection, the redistribution of MNDs was recorded: the amount of nanoparticles in the lungs decreases 3.5 fold and increases practically 3 fold in the liver. There was also registered the accumulation of MNDs in the spleen. At the same time, no change in the content of MNDs in the heart and kidneys was observed. In general, the obtained results open up the prospects for using EPR spectrometry to study the dynamics of inter-organs distribution, accumulation and elimination of MNDs after intravenous injection to the body of experimental animals.
nanodiamonds, EPR spectrometry, biological samples, blood, organs homogenates, distribution dynamics
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