In the article described investigation of complexation of biologically active agents (antibiotic topotecan, acridine dye proflavine, an analogue of vitamin B2 (riboflavin), the alkaloid caffeine), belonging to different classes of compounds using Raman spectroscopy. This study is important for explaining the mechanisms of modulation of biological activity of drugs, when they are used in the mode of complex therapy. The paper describes one technique for amplification of Raman signal, by applying a test substance to the plate with noble metals nanomaterials. A brief description of the technology for manufacturing these plates is also given. This article describes how to conduct an experiment on 3D laser scanning Raman microscope Confotec® NR500, as well as a brief analysis of some of the obtained spectra. The assumptions about the reasons for some failure of the experimental part are made and the ways of solving this problem are outlined.
Raman-spectroscopy, SERS-substrate, complexation
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