Diatoms Cylindrotheca closterium (Ehrenb.) Reimann et Lewin is able to synthesize biologically active substances: carotenoids, polyunsaturated fatty acids (PUFAs), vitamins, and others. Among all carotenoids, fucoxanthin (Fc) is the most important, which according to our data in C. closterium cells accumulates up to 2.3% of the dry mass. Thus C. closterium is advisable to grow on an industrial scale in order to obtain fucoxanthin and lipids (PUFA). For the selection of optimal conditions for the synthesis of lipids under industrial cultivation conditions, there was a need to develop an express method for determining lipids in biomass diatoms. Objective: to experimentally determine the relationship between the concentrations of fucoxanthin and total lipids in the biomass of diatom alga C. closterium (Ehrenb.) Reimann et Lewin under various cultivation conditions.We found a linear relationship between the accumulation of fucoxanthin and total lipids in cells of the diatom C. closterium under the various conditions culture. Based on this relationship, it is possible to develop an express method for the determination of lipids.
diatoms, fucoxanthin, lipids
1. D'Orazio N., Gemello E., Gammone M.A., de Girolam M., Ficoneri C., Riccioni G. Fucoxantin: A Treasure from the Sea. Marine Drugs, 2012, vol. 10, pp. 604-616.
2. Dunstan G.A., Volkman J.K., Barrett S.M., Leroil J.-M., Jeffrey S.W. Essential polyunsaturated fatty acids from 14 species of diatom (Bacillariophyceae). Phytochemistry, 1994, vol. 35, pp. 155-161.
3. Suman K., Kiran T., Devi U.K., Sarma N.S. Culture medium optimization and lipid profiling of Cylindrotheca, a lipid- and polyunsaturated fatty acid-rich pennate diatom and potential source of eicosapentaenoic acid. Botanica Marina, 2012, vol. 55, pp. 289-299.
4. Affan A., Heo S.-J., Jeon Y.-J., Lee J.-B. Optimal growth conditions and antioxidative activities of Cylindrotheca closterium. Journal of Phycology, 2009, vol. 45, pp. 1405-1415.
5. Kumar S.R., Hosokawa M., Miyashita K. Fucoxanthin: a marine carotenoid exerting anti-cancer effects by affecting multiple mechanisms. Marine Drugs, 2013, vol. 11, pp. 5130-5147.
6. Maeda H., Hosokawa M., Sashima T., Murakami-Funayama K., Miyashita K. Anti-obesity and anti-diabetic effects of fucoxanthin on diet-induced obesity conditions in a murine model. Molecular Medicine Reports, 2009, vol. 2, iss. 6. pp. 897-902.
7. Gevorgiz R.G., Zheleznova S.N., Nikonova L.L., Bobko N.I., Nehoroshev M.V. Ocenka plotnosti kul'tury fototrofnyh mikroorganizmov metodom yodatnoy okislyaemosti. Sevastopol', 2015, 31 s. [Gevorgiz R.G., Zheleznova S.N., Nikonova L.L., Bobko N.I., Nekhoroshev M.V. Estimation of the density of culture of phototrophic microorganisms by the iodate oxidation method. Sevastopol, 2015, 31 p. (In Russ.)]
8. Rukovodstvo po sovremennym biohimicheskim metodam issledovaniya vodnyh ekosistem, perspektivnyh dlya promysla i marikul'tury. Red. A.I. Agatova. Moskva: izd-vo VNIRO, 2004, 123 s. [A guide on modern biochemical methods for studying aquatic ecosystems that are promising for fishing and mariculture. Ed. A.I. Agatova. Moscow: publishing house VNIRO, 2004, 123 p. (In Russ.)]
9. Hashimoto T., Ozaki Y., Taminato M., Dass S.K., Mizuno M., Yoshimura K., Maoka T., Kanazawa K. The distribution and accumulation of fucoxanthin and its metabolites after oral administration in mice. British Journal of Nutrition., 2009, vol. 102, pp. 242-248.
10. Trenkenshu R.P. Dinamicheskaya model' biotransformacii rezervnyh i strukturnyh form biomassy mikrovodorosley v temnote. Voprosy sovremennoy al'gologii, 2016, № 2 (12), URL: http://algology.ru/967. [Trenkenshu R.P. Dynamic model of biotransformation of reserve and structural forms of microalgae biomass in the dark. Voprosy sovremennoy al'gologii , 2016, no. 2 (12), URL: http://algology.ru/967. (In Russ.)]
11. Pratiwi A.R., Syah D., Hardjito L., Panggabean L.M.G., Suhartono M.T. Fatty Acid Synthesis by Indonesian Marine Diatom, Chaetoceros gracilis. Hayati Journal of Biosciences, 2009, vol. 16, iss. 4, pp. 151-156.
