Cisplatin is a chemotherapeutic drug, successfully used in the treatment of a wide range of tumors. This drug can cause mitochondrial dysfunction and increased production of reactive oxygen spices (ROS), which leads to the damage of membrane, DNA and other important cell elements. The aim of the study was to investigate the changes in the level of ROS at different stages of cell death caused by cisplatin. We used the HeLa Kyoto cell line, the fluorescent dye DCFH-DA, the marker of apoptosis PE-Annexin V, and the vital dye 7-actinaminomycin D. Using flow cytometry, it was shown that after treatment most of viable cells are intensely stained with DCFH-DA, which indicates active oxidation processes and high level of ROS. At the stage of early apoptosis, the amount of ROS decreases as compared to the untreated cells.
cisplatin, reactive oxygen spices, apoptosis
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