Hydrogen peroxide plays a dual role in the cell: it participates in the development of free radical oxidation and functions as a signal molecule that takes part in the regulation of a variety of cellular processes. This causes a particular interest to hydrogen peroxide. HyPer is a fluorescent sensor of protein nature, which allows monitoring of the dynamic of intracellular H2O2 concentration. We create a stably transfected cell line of human epidermoid carcinoma A431-HyPer. It was experimentally proved that the performed transfection had no significant effect on the resistance of the cells to photodynamic treatment with Fotoditazin®. Using the obtained A431-HyPer cell line, a monotonous increase in the H2O2 content in the cytoplasm of the cells pre-incubated with Photoditazine was registered for half an hour after irradiation at a dose of 50 J/cm2. An increase in the H2O2 content for a relatively long time after light irradiation allows to conclude that it produced in the secondary processes developing as a result of photodynamic treatment.
photodynamic therapy (PDT), hydrogen peroxide, protein sensor HyPer, Fotoditazin
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