Nizhny Novgorod, Nizhny Novgorod, Russian Federation
Nizhny Novgorod, Nizhny Novgorod, Russian Federation
Modern strategies and approaches for biomedical diagnostics based on biohybrid photoluminescent nanomaterials are being actively developed. One of the promising areas is the synthesis of theranostic complexes for their targeted delivery and highly sensitive detection of tumor cells. In the present work we have created two-component nanocomplexes based on upconversion nanoparticles, highly effective contrast agents with unique photoluminescence properties. A non-immunoglobulin targeting protein DARPin9-29, which can selectively recognize the oncomarker HER2/neu, was used as a targeting module. A specifically binding of created complexes to the human breast adenocarcinoma SK-BR-3 cells overexpressing the HER2/neu-receptor has been shown. The obtained results allow to consider the proposed bioconjugation scheme as a promising platform for creating theranostic agents.
HER2/neu, DARPin9-29, theranostics, upconversion nanoparticles, HER2/neu, DARPin9-29
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