Cell biology occupies a special place in the study of the biological effects of microwave radiation. Cells as the main structural elements of organs and tissues, as well as intercellular interaction, which ultimately controls the processes of functioning, birth and death of organisms are always the focus of attention of specialists. The results of our previous experiments are devoted to studying the effect of microwave radiation on human cells that are under stress. Oxidative stress plays a significant role in pathological processes. In tissues, oxidative stress leads to apoptosis, which affects not only nucleated cells, but also non-nuclear cells, such as red blood cells and platelets. Platelet function is one of the main causes of vascular pathologies. However, it is not always possible to overcome platelet aggregation and prevent the development of thrombotic complications with the help of antiplatelet drugs. In this paper, one of the aspects of the application of extremely high frequencies (EHF) radiation in medical applications is considered, namely, as a factor in the correction of disturbances in the rheological properties of blood in clinical practice. By modulating the activation of platelets with the help of microwave radiation, we can thereby reject various subpopulations of platelets. In addition, EHF radiation due to the influence on the aquatic environment and the structure of platelet membranes can accelerate the process of adjusting the state of platelets. In our experiments, platelet-rich plasma (PRP) was incubated at room temperature under EHF irradiation for 30 minutes, and then the level and rate of platelet aggregation was measured. The results of our experiments show that EHF radiation reduces the degree of aggregation and the angle of inclination of the aggregatogram compared to the control with the addition of the inducer ristomycin. The addition of ethanol to the platelet medium contributed to a further decrease in the monitored parameters. It is assumed that microwave irradiation, by stimulating an increase in the rate of formation of free radicals in PRP, can regulate platelet aggregation activity. The mechanism of the observed effect associated with increased cell apoptosis is considered.
EHF radiation, intercellular interaction, platelet-rich plasma, ethanol, aggregation inducers, alcohol intoxication, mechanism of the biological action of EHF radiation
1. Liu L., Chen M., Zhao L., Zhao Q., Hu R., Zhu J., Yan R., Dai K. Ethanol Induces Platelet Apoptosis. Alcoholism, Clinical and Experimental Research, 2017, vol. 42, no 2, pp. 291-298
2. Baysan O., Kaptan K., Erinf K., Oztas Y., Coskun T., Kayir H., Uzun M., Uzbay T., Beyan C., Isik E. Chronic heavy ethanol consumption is associated with decreased platelet aggregation in rats. The Tohoku journal of experimental medicine, 2005, no 2, pp. 85-90
3. Ruf J.C. Alcohol, wine and platelet function. Biol Res, 2004, vol. 37, no 2, pp. 209-15.
4. Kazarinov K.D., Baranova O.A., Schelkonogov V.A., Chekanov A.V. Eksperimental'nye rezul'taty izucheniya effektov KVCh izlucheniya na mezhkletochnye vzaimodeystviya v plazme krovi cheloveka. Elektronnaya tehnika. SVCh-tehnika, 2019, vyp. 2, t. 545, s. 79-86. [Kazarinov K.D., Baranova O.A., Shchelkonogov V.A., Chekanov A.V. Experimental results of studying the effects of EHF radiation on intercellular interactions in human plasma. Elektronnaya tekhnika. SVCH-tekhnika, 2019, iss. 2, vol. 545, pp. 79-86. (In Russ.)]
5. Birka C., Lang A.P., Kempe S.D. Enhanced susceptibility to erythrocyte «apoptosis» following phosphate. Eur. J. Physiol, 2004, vol. 448, pp. 471-477.
6. Leytin V., Mykhaylov S., Starkey A.F. et al. Intravenous immunoglobulin inhibits anti-GPIIb-induced platelet apoptosis in a murine model of ITP. Br. J. Haematol, 2006, vol. 133, pp. 78-82.
7. Alberio L., Safa O., Clemetson K.J., Esmon C.T., Dale G.L. Surface expression and functional characterization of alpha-granule factor V in human platelets: effects of ionophore A23187, thrombin, collagen, and convulxin. Blood, 2000, vol. 95, pp. 1694-702
8. Berezovskaya G.A. Apoptoz trombocitov: prichiny nedostatochnoy effektivnosti antitrombocitarnyh preparatov. Byulleten' SO RAMN, 2011, t. 32, № 4, s. 17-27 [Berezovskaya G.A. Platelet apoptosis: causes of insufficient efficacy of antiplatelet drugs. Byulleten' SO RAMN, 2011, vol. 32, no. 4, pp. 17-27. (In Russ.)]
9. Ermolaeva E.N., Krivohizhina L.V., Kantyukov S.A., Surina-Marysheva E.F. Svobodnoradikal'noe okislenie v intaktnyh i aktivirovannyh trombocitah. Fundamental'nye issledovaniya, 2014, № 7-1, s. 61-65. [Ermolaeva E.N., Krivokhizhina L.V., Kantyukov S.A., Surin-Marysheva E.F. Free radical oxidation in intact and activated platelets. Fundamental'nyye issledovaniya, 2014, no. 7-1, pp. 61-65. (In Russ.)]
10. Sobotková A., Mášová-Chrastinová L., Suttnar J. Antioxidants change platelet responses to various stimulating events. Free Radic Biol Med., 2009, vol. 47, no. 12, pp. 1707-1714.
11. Terent'eva V.A., Sveshnikova A.N., Panteleev M.A. Biofizicheskie mehanizmy kontaktnoy aktivacii svertyvaniya plazmy krovi. Biofizika, 2017, t. 62, № 5, s. 909-919. [Terentyeva V.A., Sveshnikova A.N., Panteleev M.A. Biophysical mechanisms of contact activation of blood plasma coagulation. Biofizika, 2017, vol. 62, no. 5, pp. 909-919. (In Russ.)]
12. Obydennyy S.I., Sveshnikova A.N., Ataullakhanov P.M. Dynamics of calcium spiking, mitochondrial collapse and phosphatidylserine exposure in platelet subpopulations during activation. Journal of Thrombosis and Haemostasis, 2016, vol. 14, no. 9, pp. 1867-1881.
13. Hartwig J.H. The platelet: form and function. Seminars in hematology, 2006, vol. 43, no 1, pp. 94-100. DOI:https://doi.org/10.1053/j.seminhematol.2005.11.004.
14. Kazarinov K.D., Borisenko G.G., Polnikov I.G. Deystvie KVCh nizkoy intensivnosti na okislitel'nye processy. Nauchnye trudy VIII Mezhdunarodnogo kongressa «Slabye i sverhslabye polya i izlucheniya v biologii i medicine», SPb: 2018, c. 43 [Kazarinov KD, Borisenko G.G., Polnikov I.G. Effect of low-intensity EHF on oxidative processes. Nauchnyye trudy VIII Mezhdunarodnogo kongressa «Slabyye i sverkhslabyye polya i izlucheniya v biologii i meditsine», St. Petersburg: 2018, p. 43. (In Russ.)]
15. Kazarinov K.D. Issledovanie membranotropnoy aktivnosti EMI v shirokom diapazone dlin voln. Elektronnaya tehnika. Ser. 1. SVCh - tehnika, 2018, Vyp. 2, S. 62-75 [Kazarinov K.D. Investigation of the membranotropic activity of electromagnetic radiation in a wide range of wavelengths. Elektronnaya tekhnika. Ser. 1. SVCH - tekhnika, 2018, vol. 2, pp. 62-75. (In Russ.)]
