Nizhny Novgorod, Nizhny Novgorod, Russian Federation
Photodynamic therapy is an intensively developing method of treating oncological diseases based on selective accumulation of a photosensitizer in a tumor and its subsequent local activation with visible light in the presence of oxygen, leading to the formation of free radicals that are toxic to surrounding cells. Currently, the method of photodynamic therapy has a number of unsolved problems, among which we can highlight lack of tool for early assessment of the effectiveness of treatment and the empirical nature of the selection of light irradiation dose. It is known that photodynamicaly induced cell death is accompanied by a significant increase in intracellular viscosity. One way to assess intracellular viscosity is to use fluorescent molecular rotors - compounds whose quantum yield and fluorescence lifetime strongly depend on the medium viscosity. It has previously been shown that tetrakis(4-fluorophenyl)tetracyanoporphyrazine is a fluorescent molecular rotor and agent for photodynamic therapy. In the present work, we evaluated dose-dependent changes in the viscosity properties of tumor cells under photodynamic treatment with porphyrazine using the time-resolved microscopy method. We have shown that the response of tumor cells to porphyrazine-based photodynamic treatment is dose-dependent that is manifested in in the time of onset and in the degree of increase in intracellular viscosity. In turn, the viscosity increase correlates with destructive changes in the cell morphology.
FLIM, porphyrazine, photodynamic therapy, molecular rotor, viscosity, FLIM
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