The paper presents the results of a study of the microcirculatory bed and parameters of oxidative blood metabolism of intact animals exposed to prolonged inhalation of nitric oxide at concentrations of 20 ppm, 50 ppm and 100 ppm. The experiment was performed on Wistar rats. No inhalations were carried out within 30 days. The state of blood microcirulation and oxidative metabolism was assessed 30 days later and the recovery period after the cancellation of oxidative loads NO (60 days). Laser Doppler flowmetry was used to study the functioning of skin microvessels in terms of perfusion. The intensity of lipid peroxidation was studied in blood plasma and erythrocytes using the method of induced biochemiluminescence and the level of Malon dialdehyde. The activity of superoxide dismutase was determined in erythrocyte hemolysate. It was found that the optimal concentration for inhalation use of NO is 20 ppm, which was used to increase perfusion, as well as the maximum increase in total antioxidant activity in 30 days and normalization of lipid peroxidation in the blood 60 days after use of NO. High concentrations of nitric oxide (50 ppm and 100 ppm) had an initiating effect on microcirculation and lipid peroxidation in erythrocytes and blood plasma after the cancellation of NO exposure for 60 days, activating the catalytic properties of superoxide dismutase.
nitric oxide, inhalation, microcirculation, lipid peroxidation, Malon dialdehyde, superoxide dismutase
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