The estimation of limit productivity and coefficient of carbon absorption by Dunaliella viridis culture grown during bubbling of suspension with the help of aquarium spray was carried out. Provided that the content of carbon dioxide in the air is 0.04 %, the limit productivity of the culture of microalgae is 0.622 g SV/(l∙day), while the minimum volume of air that must be fed to the photobioreactor for growing 1 g of biomass is 2310 L. With an increase in the proportion of carbon dioxide in the air by 0.01%, the value of the limit productivity increases by 1.35 times. It was experimentally shown that at the surface illumination of a photobioreactor of 10 klx, the air purge rate of 1 l / l per minute, the absorption carbon coefficient of D. viridis culture is 18 %, and the number of cells that can grow on one gram of carbon is 0.74 million/ml.a comparative assessment of the absorption carbon coefficient of different types of microalgae was carried out. Changes in the value of this parameter were observed in D. viridis, P. purpureum and D. salina , which can be explained by different light conditions. The generalized parameters of microalgae growth defined in this paper can be used to optimize their cultivation with different methods of carbon supply.
Dunaliella viridis, storage culture, maximum productivity, carbon dioxide, air
1. Borovkov A.B. Zelenaya mikrovodorosl' Dunaliella salina Teod. (obzor). Ekologiya morya, 2005, t. 67, s. 5-17. @@[Borovkov A.B. Green microalga Dunaliella salina Teod. (review). Ecologiya morya, 2005, vol. 67, pp. 5-17. (In Russ.)]
2. Masyuk N.P. Morfologiya, sistematika, ekologiya, geograficheskoe rasprostranenie roda Dunaliella Teod. Kiev: Naukova dumka, 1973, 487 s. @@[Masuk N.P. Morphology, systematics, ecology, geographical distribution of the genus Dunaliella Teod. Kyev: Naukova dumka, 1973, 487 p. (In Russ.)]
3. Oren A. A hundred years of Dunaliella research: 1905-2005. Saline Systems, 2005, URL: http://www.salinesystems.org/content/1/1/2. DOI:https://doi.org/10.1186/1746-1448-1-2.
4. Ben-Amotz A., Shaish A., Avron M. Mode of action of the massively accumulated, β-carotene of Dunaliella bardawil in protecting the alga against damage by excess irradiation. Plant Physiol, 1989, vol. 91, no. 3, pp. 1040-1043.
5. Kozhemyaka A.B. Zavisimost' koncentracii organicheskogo veschestva v kletke ot ee ob'ema dlya chernomoskih vidov Bacillariophyta. Morskoy ekologicheskiy zhurnal, 2014, t. 8, № 1, s. 35-43. @@[Kozhemyaka A.B. The dependence of the concentration of organic matter in the cell on its volume for the Black Sea species of Bacillariophyta, Morskoy ekologicheskiy zhurnal, 2014, vol. 8, no. 1, pp. 35-43. (In Russ.)]
6. Lelekov A.S., Gudvilovich I.N., Gevorgiz R.G., Trenkenshu R.P., Badisova A.O. Ocenka koefficienta absorbcii ugleroda kul'turoy Porphyridium purpureum (Bory) Ross. Sbornik materialov Vseros. nauch.-prakt. konf. k 145-letiyu Sevastopol'skoy biologicheskoy stancii (Sevastopol', 19-24 sent. 2016 g.). Sevastopol': EKOSI-Gidrofizika, 2016, t. 3, s. 404-407. @@[Lelekov A.S., Gudvilovich I.N., Gevorgiz R.G., Trenkenshu R.P., Badisova A.O. Estimation of carbon absorption coefficient by Porphyridium purpureum (Bory) Ross culture. Collection of materials Vseros. scientific-practical conf. to the 145th anniversary of the Sevastopol Biological Station (Sevastopol, September 19-24, 2016). Sevastopol: ECOSI-Hydrophysics, 2016, vol. 3, pp. 404-407. (In Russ.)]
7. Trenkenshu R.P., Belyanin V.N. Vliyanie elementov mineral'nogo pitaniya na produktivnost' vodorosli Platymonas viridis. Biologiya morya, 1979, t. 51, s. 41-46. @@[Trenkenshu R.P., Belyanin V.N. The effect of mineral nutrients on the productivity of the alga Platymonas viridis. Bioligiya morya, 1979, vol. 51, pp. 41-46. (In Russ.)]
8. Trenkenshu R.P., Lelekov A.S., Borovkov A.B., Novikova T.M. Unificirovannaya ustanovka dlya laboratornyh issledovaniy mikrovodorosley. Voprosy sovremennoy al'gologii, 2017, № 1 (13), URL: http://algology.ru/1097 @@[Trenkenshu R.P., Lelekov A.S., Borovkov A.B., Novikova T.M. The unified installation for laboratory research of microalgae. Issues of modern algology, 2017, no. 1 (13), URL: http://algology.ru/1097 (In Russ.)]
9. Memetshaeva O.A. Reproduktivnaya aktivnost' kletok Dunaliella viridis Teod. pri raznom uglerodnom obespechenii v nakopitel'noy kul'ture. Voprosy sovremennoy al'gologii, 2018, № 3 (18), URL: http://algology.ru/1369 @@[Memetshaeva O.A. Reproductive activity of Dunaliella viridis Teod cells. with different carbon supply in the funded culture. Issues of modern algology, 2018, no. 3 (18), URL: http://algology.ru/1369 (In Russ.)]
10. Doney S.C., Fabry V.J., Feely R.A., Kleypas J.A. Ocean Acidification: the other CO2 problem. Annu. Rev. Mar. Sci., 2009, vol. 1, pp. 169-192.
11. Gudvilovich I.N., Borovkov A.B. Produktivnost' mikrovodorosli Dunaliella salina Teod. pri razlichnyh sposobah vneseniya uglekislogo gaza v kul'turu. Morskoy biologicheskiy zhurnal, 2017, t. 2, № 2. c. 34-40. DOI:https://doi.org/10.21072/mbj.2017.02.2.03. @@[Gudvilovich I.N., Borovkov A.B. Microalgae productivity Dunaliella salina Teod. with various methods of introducing carbon dioxide into the culture. Morskoy ekologicheskiy zhurnal, 2017, vol. 2, no. 2, pp. 34-40. DOI:https://doi.org/10.21072/mbj.2017.02.2.03. (In Russ.)]
12. Pronina N.A. Organizaciya i fiziologicheskaya rol' SO2-koncentriruyuschego mehanizma pri fotosinteze mikrovodorosley. Fiziologiya rasteniy, 2000, t. 47, № 5, s. 801-810. @@[Pronina N.A. Organization and physiological role of the CO2-concentrating mechanism in microalgae photosynthesis. Fiziologiya rasteniy, 2000, vol. 47, no. 5, pp. 801-810. (In Russ.)]
13. Gevorgiz R.G., Zheleznova S.N., Zozulya Yu.V., Uvarov I.P., Lelekov A.S., Nehoroshev M.V. Promyshlennaya tehnologiya proizvodstva fukoksantina na osnove intensivnoy kul'tury morskoy diatomei Cylindrotheca closterium (Ehrenberg) Reimann & Lewin v gazovihrevom fotobioreaktore. Rossiyskiy bioterapevticheskiy zhurnal, 2016, t. 15, № 1, s. 22. @@[Gevorgiz R.G., Zheleznova S.N., Zozulya U.V., Uvarov I.P., Lelekov A.S., Nekhoroshev M.N. Industrial technology for the production of fucoxanthin based on the intensive culture of the marine diatom Cylindrotheca closterium (Ehrenberg) Reimann & Lewin in a gas-vortex photobioreactor. Russian Journal of Biotherapy, 2016, vol. 14, no, 1, p. 22. (In Russ.)]
