The aim of this work is to study the influence of γ - rays on yeast cells by recording changes in the parameters of fluorescence of pyrene in membranes. By using the method of fluorescent zonde was carried out the estimation of microviscosities of membranes in yeast cells under the influence of γ-irradiation at dose 5-150 Gy. According to the spectra of pyrene fluorescence has been determined the microviscosity of lipid bilayer and zones of protein-lipid contacts. It was shown that different doses differently change such physical parameters of the membrane as microviscosity and polarity of the lipid bilayer. Based on data, it was shown that, after the irradiation of yeast cells, the change of viscosity is a reflection of adaptive structural-functional rearrangements. The totality of the obtained data suggests that, adaptation processes at high radiation dose (75-100 Gy.) in yeast cells, obviously, end at an earlier time.
microviscosity, yeast cells, γ-radiation, pyrene, polarity
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