DECOMPOSITION OF THE RED AREA OF THE ABSORPTION SPECTRUM - THE INITIAL METHOD OF EVALUATING THE PIGMENTAL COMPOSITION OF MICROALGAE
Abstract and keywords
Abstract (English):
The paper describes the choice of a logical model, as well as the stabilization mode for automatic control systems of microalgae growth. The absorption spectra of the culture and acetone extracts of the microalgae Spirulina platensis and Dunaliella salina were quantitatively described by three Gauss curves in the range from 550 to 700 nm. The normalized spectra of cultures of the microalgae Spirulina platensis and Dunaliella salina are also described, and the difference in the absorption spectra in the region of 550-700 nm is determined. Based on the results of approximating the difference in the absorption spectra of the cultures, a model of chlorophyll b was obtained. Based on the data obtained, as well as their comparison, it was concluded that this model meets the requirements for the designed instrument base.

Keywords:
spirulina, chlorophyll a, absorption spectra, Gaussians, system of automatic control of microalgae growth
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References

1. Kopytov Yu.P. i dr. Metodika kompleksnogo opredeleniya biohimicheskogo sostava mikrovodorosley. Al'gologiya, 2015. @@[Kopytov Yu.P. et al. Methodology for the complex determination of the biochemical composition of microalgae. Algology, 2015. (In Russ.)]

2. Sid'ko F.Ya., Belyanin V.N. Rost i effektivnost' fotosinteza hlorelly pri preryvistom obluchenii. Doklady Akademii nauk SSSR. Izd-vo Akademii nauk SSSR, 1976, t. 230, № 4-6, s. 998. @@[Sidko F.Ya., Belyanin V.N. Growth and efficiency of chlorella photosynthesis under intermittent irradiation. Doklady of the USSR Academy of Sciences. Publishing House of the Academy of Sciences of the USSR, 1976, vol. 230, no. 4-6, pp. 998. (In Russ.)]

3. Trenkenshu R.P., Belyanin V.N. Vliyanie elementov mineral'nogo pitaniya na produktivnost' vodorosli Platymonas viridis Rouch, 1979. @@[Trenkenshu R.P., Belyanin V.N. Influence of mineral nutrition elements on the productivity of the alga Platymonas viridis Rouch, 1979. (In Russ.)]

4. Jeffrey S.W. Data for the identification of 47 key phytoplankton pigments. Phytoplankton pigments in oceanography, 1997, pp. 449-559.

5. Küpper H., Seibert S., Parameswaran A. Fast, sensitive, and inexpensive alternative to analytical pigment HPLC: quantification of chlorophylls and carotenoids in crude extracts by fitting with Gauss peak spectra. Analytical chemistry, 2007, vol. 79, no. 20, pp. 7611-7627.

6. Chernyshev D.N., Borovkov A.B. Razdelenie spektra pogloscheniya acetonovogo ekstrakta Dunaliella salina. Aktual'nye voprosy biologicheskoy fiziki i himii, 2016, № 1-1, s. 51-56. @@[Chernyshev D.N., Borovkov A.B. Separation of the absorption spectrum of the acetone extract of Dunaliella salina. Actual problems of biological physics and chemistry, 2016, no. 1-1, pp. 51-56. (In Russ.)]

7. Bidigare R.R. et al. In-vivo absorption properties of algal pigments. Ocean Optics X. - International Society for Optics and Photonics, 1990, vol. 1302, pp. 290-303.

8. Küpper H., Spiller M., Küpper F.C. Photometric method for the quantification of chlorophylls and their derivatives in complex mixtures: fitting with Gauss-Peak spectra. Analytical Biochemistry, 2000, vol. 286, no. 2, pp. 247-256.


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